摘要
目的:建立白头翁药材高效液相色谱指纹图谱,并比较了不同产地白头翁中白头翁皂苷B4含量的差异。方法:采用Agilent ZORBAX SBC18(4.6 mm×250 mm,5μm)色谱柱;乙腈(A)和水(B)为流动相,流速1.0 ml/min;柱温40℃;检测波长201 nm。结果:十批白头翁样品高效液相色谱指纹图谱具有一定的相似性;不同产地白头翁中白头翁皂苷B4的含量有差异。结论:本法准确、可靠,建立的白头翁高效液相色谱指纹图谱可结合白头翁皂苷B4含量测定用于白头翁药材的质量控制。
Objective： To establish the HPLC fingerprint and compare the content of pulchinenoside B4 of Pulsatillae Radix from different areas. Methods： RP - HPLC method was applied and the conditions were as follows： Column： Agilent ZORBAX SBCiscolumn （4.6mm×250 mm, 5 μm）; mobile phase： acetonitrile （A） and H20 （B）, flow-rate： 1.0 mL/min; column temperature： 40℃ ; the detection wavelength was 201nm. Results： There was a certain similarity in the fingerprint of the ten samples of Pulsatillae Radix. The difference on the content of pulchinenoside B4 was obvious. Conclusion： The method is accurate, reliable and the fingerprint can be combined with content determination of pulchineneside B4 in the quality^control of Pulsatillae Radix.
出处
《宜春学院学报》
2011年第12期130-133,共4页
Journal of Yichun University
基金
安徽省高校省级自然科学研究项目(No.KJ2011Z083)
关键词
白头翁
高效液相色谱
指纹图谱
白头翁皂苷B4
Ptdsatillae Radix
High performance liquid chromatography
Fingerprint
Pulchinenoside B4
