摘要
目的对链霉菌FIM-04-806的次级代谢产物,含双噁唑环的大环双内酯化合物FW-04-806进行发酵条件研究。方法采用单因素试验进行培养基优化,探讨装液量、初始pH值等发酵参数的影响,同时逐级放大进行中试验证。结果确定了最佳培养条件:种子培养基为葡萄糖3%,蔗糖3%,玉米浆粉2%,CaCO3 0.75%,pH7.5;发酵培养基为可溶性淀粉3%,葡萄糖2%,黄豆粉1%,玉米浆粉2%,CaCO3 0.7%,pH7.5,接种量10%。优化后的摇瓶效价较初始水平提高了7倍以上,并在100L自动发酵罐及1.5吨发酵罐上得到验证。结论 FW-04-806优化的发酵条件为其工业化生产奠定了基础。
Objective To study the fermentation conditions of FW-04-806, a macrolide dilactone compound with two oxazole ring, which was produced by Streptomyces sp. FIM-04-806. Methods Several basic fermentation conditions were designed in shake flasks, such as inoculums size, broth volum and the initial pH of the fermentation process, further optimization of the culture conditions was perfomed in the fermentation scale-up process. Results The yield of FW-04-806 from Streptomyces sp. FIM-04-806 was seven times increased under the optimized conditions： seed medium contains 3% glucose, 3% sucrose, 2% corn syrup powder, CaCO3 0.75%, pH7.5; fermentation medium contains 3% starch, 2% glucose, 1% soybean flour, 2% corn syrup powder, CaCO3 0.7%, pHT.5; and the cultivation parameters were verified in 100L automatic bioreactor and 1.5 ton fermenter tank. Conclusion It had established the foundation for producing efficiently FW-04-806 on industrial scale.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2012年第1期45-48,65,共5页
Chinese Journal of Antibiotics
基金
国家科技重大专项课题(2009ZX09103-004)
福建省自然科学基金项目(2009J01075)
