摘要
利用玉米浆、豆粕作为药食真菌蛹虫草、松茸混菌共酵的廉价培养基,不仅可以提高废料的利用价值,而且可以降低蛹虫草、松茸混菌共酵获得胞外多聚物的生产成本。以培养基中玉米浆、豆粕浓度作为考察因子,在单因素试验的基础上,进行了中心组合试验,采用响应面分析法优化了蛹虫草、松茸混菌共酵的培养基配方。试验结果表明,豆粕对蛹虫草、松茸混菌共酵中胞外多聚物产率的影响非常显著,最佳培养基配方为:玉米浆浓度9.18%,豆粕浓度13.04%,在该最佳培养基条件下进行验证试验,胞外多聚物产率可达30.65 g/L,与模型的预测值30.26 g/L基本一致,表明模型可较好地预测蛹虫草、松茸混菌共酵中胞外多聚物的产率。
The aim of this work was to reduce the production costs and make full use of industrial and agricultural waste on the exo-biopolymer production by co-cultivating the medicinal and edible fungus Tricholoma matsutake and Cordyceps Militaris using corn steep liquor and soybean meal. The concentration of corn steep liquor and soybean meal in the medium was investigated by means of single factor tests and Response Surface Methodology （RSM） based on a five-level two-factor central composite design. Optimized values of the factors were determined to be corn steep liquor 9.176 % and soybean meal 13.044 % and a maximum exo- biopolymer production of 30.263 19 g/L was obtained which accorded with the predicted value of 30.26 %. This showed that the model can be used to simulate and predict the experimental exo-biopolymer yield.
出处
《食品研究与开发》
CAS
北大核心
2008年第6期20-24,共5页
Food Research and Development
基金
吉林大学国家级生物实验教学示范中心设计创新实验基金一类资助项目(ZXCX2006001)
关键词
响应面法
共酵
胞外多聚物
玉米浆
豆粕
Response Surface Methodology
co-cultivation
exo-biopolymer
corn steep liquor
soybean meal
