摘要
对前期筛选得到的咪唑立宾产生菌Eupenicillium sp.E-UN41,通过单因素筛选结合正交试验进行发酵培养基优化,得到最佳配方:可溶性淀粉3.0%,黄豆粉3.5%,K2HPO40.005%,MgSO40.05%,CaCO30.2%.对摇瓶条件下液体发酵的主要影响因子温度、转速、初始pH值等进行实验探讨,确定最佳培养参数:初始pH值7.0,种子菌龄48 h,装液量50 mL/500 mL,接种量2%,摇床转速250 r.min-1,发酵温度28℃;最佳发酵时间为7 d,其发酵效价相比对照提高38%.
The single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and conditions of Eupenicillium sp.E-UN41.The results indicated that the constituents of medium,the filling volume of shaking flask,the rotation speed and the temperature were the major factors that affected the yield of mizoribine（MZ）.The composition of soluble starch 3.0%,soybean meal 3.5%,K2HPO4 0.005%,MgSO4 0.05% and CaCO3 0.2% was selected to be the medium formula,and the optimal fermentation parameters were as follows： the filling volume 50 ml for a 500 ml shake flask,initial pH at 7.0,shaker＇s rotating speed 250 r·min-1,inoculum size 2%,temperature 28 ℃,cultivation time of seed 48 h and fermentation time 7 d,and the titer of mizoribine was 38% higher than that in the control.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第6期970-974,共5页
Journal of Fuzhou University(Natural Science Edition)
基金
国家"十一五""重大新药"创制资助项目(20102X09401-403)
福建省科技重点资助项目(2009R10003-3)
关键词
咪唑立宾
正青霉菌
单因素试验
正交试验
发酵条件优化
mizoribine
Eupenicillium
single factor experiment
orthogonal experimnet
fermentation conditions optimization
