摘要
目的运用代谢工程手段对龟裂链霉菌工业菌(Industrial Streptomyces rimosus,SRI)进行基因改造,提高土霉素(oxytetracycline,OTC)产量。方法利用pKC1139质粒阻断SRI基因组中葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehydrogenase)编码基因zwf1。结果筛选得到一株OTC高产突变株,将突变株与原始菌株进行发酵,发现OTC产量比原始菌株提高了36.2%。结论 SRI基因组中zwf1基因的缺失使细胞合成土霉素的能力增强;龟裂链霉菌中初级代谢关键基因调控会影响次级代谢。
Objective To enhance oxytetracyclin （OTC） production in Industrial Streptomyces rimosus by gene disruption according to metabolic engineering principle. Methods Disrupt zwfl gene encoding glucose-6-phosphate dehydrogenase in SRI strain using plasmid pKCl139. Results A mutant strain was successfully constructed and screened which produced OTC 36.2% more than parent strain. Conclusion The results suggest that it is an effective way to enhance OTC productivity in an industrial strain by zwfl knock-out. The regulation of key genes in primary metabolic affects secondary metabolic in S. rirnosus a lot.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2012年第1期35-38,69,共5页
Chinese Journal of Antibiotics
基金
生物反应器国家重点实验室开放课题(2060204)
国家支撑项目<抗生素大规模生产关键技术创新-抗生素发酵工艺与装备集成创新研究>(2007BAI26B02)
