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脂联素对高糖环境下人心肌细胞增殖和凋亡的干预作用 被引量：4

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摘要 目的探讨脂联素(Adiponectin,ADPN)对高糖环境下人心肌细胞增殖、凋亡的影响,揭示脂联素在糖尿病心肌病变中的保护作用。方法将人心肌细胞用5.5mmol/L葡萄糖(正常对照组)、30.0mmol/L葡萄糖(高糖组)、30.0mmol/L+2.5μg/mlADPN(脂联素组)分别培养24h,48h,72h后,运用MTT方法检测细胞增殖,Westernblotting检测凋亡相关蛋白Bax,Bcl-2的表达,用荧光探针DCFH-DA检测细胞内ROS水平,观察细胞凋亡。结果①高糖组人心肌细胞增殖被抑制(P<0.05),ROS水平、细胞凋亡增加,并呈时间依赖性。②高糖组人心肌细胞,脂联素(2.5μg/ml)干预后,细胞增殖接近正常对照组,ROS水平下降,延缓了细胞凋亡。高糖环境下随着时间的延长,可引起心肌细胞增生肥大,诱导其凋亡;脂联素可促进细胞增殖,延缓细胞凋亡。
作者 孙霞 李兴
机构地区 山西医科大学第二医院内分泌科
出处 《中国现代药物应用》 2011年第22期79-82,共4页 Chinese Journal of Modern Drug Application
基金 山西省归国留学基金(项目编号:2009-75)
关键词 脂联素 心肌细胞 氧化应激 细胞凋亡
分类号 R587.2 [医药卫生—内分泌]
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参考文献3

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共引文献33

同被引文献46

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中国现代药物应用
2011年 第22期

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