摘要
目的探讨球状脂联素在抑制波动性高血糖诱导人脐静脉内皮细胞凋亡中的机制。方法不同条件下体外培养人脐静脉内皮细胞5天。实验分为对照组(葡萄糖5.5 mmol/L)、高糖组(葡萄糖25 mmol/L)、葡萄糖交替组(葡萄糖5.5/25 mmol/L,每8 h更换培养液一次)、高渗组(甘露醇25 mmol/L)和高渗交替组(甘露醇5.5/25 mmol/L,每8 h更换培养液一次)。葡萄糖交替组中部分细胞用不同浓度(0、0.5、1.0和3 mg/L)球状脂联素干预,用脂联素受体1特异性小干扰RNA作用内皮细胞。采用流式细胞仪检测细胞凋亡及RT-PCR检测脂联素受体1和受体2的mRNA表达。结果与对照组比较,高糖组和葡萄糖交替组细胞凋亡明显增加,脂联素受体1 mRNA的表达显著降低(P<0.01)。与高糖组比较,葡萄糖交替组细胞凋亡显著增加,脂联素受体1 mRNA的表达显著降低(P<0.01)。不同浓度(0、0.5、1.0和3 mg/L)球状脂联素作用内皮细胞,发现3 mg/L球状脂联素能显著抑制内皮细胞凋亡(P<0.01),并能显著拮抗波动性高血糖诱导的脂联素受体1 mRNA表达的下降(P<0.01)。不同组间内皮细胞脂联素受体2 mRNA表达差异无显著性。siRNA作用内皮细胞后球状脂联素这种抑制凋亡作用显著减弱(P<0.01)。结论与持续性高血糖条件比较,波动性高血糖显著降低人脐静脉内皮细胞脂联素受体1 mRNA的表达,而对脂联素受体2 mRNA的表达无影响。球状脂联素可能通过脂联素受体1拮抗波动性高血糖诱导的人脐静脉内皮细胞凋亡。
Aim To study the mechanism of human umbilical vein endothelial cells（HUVEC） apoptosis induced by fluctuated hyperglycemia. Methods HUVEC were cultured in different conditions： control group,high glucose group,alternating high glucose group,high osmotic group,and alternating high osmotic group.Alternating high glucose groups were affected by different concentrations of globular adiponectin（gAD）,or by small interfering RNA（siRNA）.Annexin Ⅴ-FITC/PI double staining was used to detect apoptosis.RT-PCR was used to detect adiponectin receptor 1（AdipoR1） mRNA and adiponectin receptor 2（AdipoR2） mRNA. Results Compared with control group,cells apoptosis was increased,and expression of AdipoR1 mRNA was decreased in both high glucose group and alternating high glucose group（P0.01）.Cells apoptosis was increased,and expression of AdipoR1 mRNA was decreased in alternating high glucose group compared with high glucose group（P0.01）.3 mg/L gAD protected against cell apoptosis compared with groups without gAD（P0.01）.gAD counteracted the decreasing expression of AdipoR1 mRNA induced by fluctuated hyperglycemia（P0.01）.But expression of AdipoR2 mRNA had no difference among them. Conclusion That gAD protected cell apoptosis induced by fluctuated hyperglycemia is associated with AdipoR1,but is not associated with AdipoR2.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2010年第7期523-526,共4页
Chinese Journal of Arteriosclerosis
关键词
细胞凋亡
人脐静脉内皮细胞
脂联素受体1
波动性高血糖
球状脂联素
Cell Apoptosis
Human Umbilical Vein Endothelial Cell
Adiponectin Receptor-1
Fluctuated High Glucose
Globular Adiponectin
