摘要
目的:初步研究大孔树脂纯化复方四叶减肥汤中总黄酮成分的工艺条件和参数。方法:以静态吸附试验、动态吸附试验、比吸附量、比洗脱量等为研究指标,比较不同大孔树脂纯化复方中总黄酮的性能,对树脂纯化工艺进行正交优选。结果:AB-8型树脂为优选树脂,最佳工艺条件为:树脂(g)-提取液(mL)比例为1.2∶1上柱,色谱柱径高比1∶12,水洗至无色,80%乙醇洗脱液收集量为7 BV。结论:该工艺稳定性良好,纯化后的总黄酮质量分数达85%以上。
Objective：To investigate purification process of total flavonoids from four-leaf diet decoction with macroreticular resin. Methods： By using the static absorption tests and dynamic absorption tests with different types of macroreticular resins, the better parameters were found and the orthogonal design were used in purified craft. Results：AB-8 resin showed the best absorption property with the following technological conditions： the ratio of resin （g） - extract （mL） is 1.2： 1, the ratio of column diameter to height was 1 ： 12, then washed with water to colorless, and the volume of 80% ethanol was 7 BV. Conclusion ： The process is stable anti the purity of total flavones is up to 85%.
出处
《中药材》
CAS
CSCD
北大核心
2011年第10期1612-1614,共3页
Journal of Chinese Medicinal Materials
基金
安徽新华学院校级自然项目(2009zr007)
关键词
分离纯化
大孔树脂
复方四叶减肥汤
Separation and purification
Macroretieular resin
Four-leaf diet decoction
