摘要
１３ 个供试的中国和英国大麦黄花叶病毒（ＢａＹＭＶ） 分离物，经ＲＮＡ１ 和ＲＮＡ２ 全基因组不同区域ＤＮＡ片段单链构象多态性分析（ＳＳＣＰ） ，外壳蛋白基因和ＲＮＡ２ ７０ｋＤ基因５＇端７０５ 碱基序列分析，以及此７０５ 碱基ＤＮＡ 片段限制性内切酶图谱分析结果，它们的ＲＮＡ１ 和ＲＮＡ２ 彼此无一相同，其中ＲＮＡ２ 变异比ＲＮＡ１ 更大。由于变异十分复杂，且没有规律性，因而当前通用的分子生物学技术尚不能简单地用于ＢａＹＭＶ 致病性分化或株系区分和检测。
DNA fragments amplified from the bipartite genome of thirteen Chinese and UK isolates of barley yellow mosaic bymovirus(BaYMV) by RT-PCR were analysed by single-strand conformation polymorphism(SSCP). Variations in the coat protein gene and the 5'-terminal part(c. 705bp) of the 70kD regions of RNA2 of these BaYMV isolates were also revealed by sequencing and restriction mapping. The results indicated that none of all thirteen isolates was identical each other at the molecular level. Differences in RNA2 were greater than those in RNA1. The variations were very complicated so that none of modern molecular techniques could simply correlate the variations at nucleotide level with pathogenicity or strain differentiation.
出处
《病毒学报》
CAS
CSCD
北大核心
1999年第4期339-347,共9页
Chinese Journal of Virology
基金
欧共体ISC项目
国家自然科学基金
浙江省青年科技人材专项资金
关键词
大麦黄花叶病毒
分子变异
序列分析
RT-PCR
SSCP
Barley yellow mosaic bymovirus
Molecular variation
Single-strand conformation polymorphism
Sequencing
Restriction mapping
