摘要
阻塞大脑中动脉２ ｈ，再灌流０．５～４８ ｈ 制成脑缺血模型，用免疫组织化学技术观察了脑缺血后白细胞共同抗原在脑组织的表达。结果表明：白细胞共同抗原阳性的小胶质细胞或巨噬细胞呈圆形，未见分支型小胶质细胞，阳性的小胶质细胞于再灌流３ ｈ 开始出现，再灌流１２ ｈ 数量显著增多，并且胞质、胞核均为强阳性；阳性的小胶质细胞分布于纹状体、下丘脑、杏仁核处及皮质与外囊相连处，完全坏死区无阳性小胶质细胞；此外，同侧微血管强表达白细胞共同抗原；同侧大脑皮质Ⅳ、Ⅴ、Ⅵ层锥体细胞、丘脑、海马形态正常的神经元亦强表达白细胞共同抗原，但核固缩的神经元白细胞共同抗原则呈阴性。本实验推测白细胞共同抗原可能有保护神经元的作用，并可能是神经元与小胶质细胞之间的信息传递分子。
Male SD rats (n=30) were subjected to 2 hours of middle cerebral artery occlusion and were killed at various times of reperfusion (0.5～48 h), CD45 expression in brain was investigated. CD45 positive microglia were round cells, ramified microglia were not found. Microglia were first present at 3 hours of reperfusion. The number of microglia was dramatically increased at 12 hours of reperfusion. Microglia expressed dense nuclear and cytoplasmic CD45 immunoreactivity. Microglia were scattered in the striatum, hypothalamus, amygdaloid nucleus and the junction between cerebral cortex and external capsule. Microglia were not present in pannecrotic area. In addition, ipsilateral microvessels strongly expressed CD45, CD45 expression was strongly induced in pyramidal neurons of cortical layer, the larger neurons of striatum and hypothalamus. The nucleus shape and hematoxylin staining of CD45 positive neurons were normal. The neurons exhibiting nuclear shrinkage didn't express CD45. The results suggested that CD45 may play a role in protecting neurons and CD45 molecule is possiblly a signal communicating molecule between micoglia and neurons. (Figures 1～13 on plate 65)
出处
《神经解剖学杂志》
CSCD
北大核心
1999年第4期373-376,共4页
Chinese Journal of Neuroanatomy
关键词
CD45
小胶质细胞
神经元
大脑中动脉阻塞
大鼠
CD45
immunohistochemistry
microglia
neuron
reversible middle cerebral artery occlusion
rat
