摘要
用免疫组化方法,对大鼠暂时性全脑缺血诱导的c-fos原癌基因蛋白(Fos)在海马表达的分布,强度和时间过程等特征进行了观察.结果表明:缺血40min再循环后2h,首先在海马齿状回转折部的颗粒细胞和下托诱导出Fos表达,5～8h达到高峰,8h后锐减并逐步消失.CA4和CA3区的Fos表达在缺血再循环后3h出现,5h达到高峰,持续至24h消失.而在CA2和CA1区,Fos表达最弱,最晚(5h才出现),持续32～48h后消失.Fos在海马表达的强弱和时间发展过程依次为齿状回、下托→CA4、CA3→CA2、CA1区.这种特征性表达可能与介导缺血损伤后强啡肽、神经生长因子等晚期基因的表达有关.
The characteristics of distribution, strength and time course of Fos expression in the rat hippocampus following transient global cerebral ischemia were investigated by using immunocytochemical method. The results showed that Fos expression was first induced in the dentate gyrus(DG) and subiculum, which appeared at 2h, peaked at 5-8h and decayed at 8h after global ischemia and recirculation (GIR). Fos induction in the CA3 and CA4 fields appeared at 3h, peaked at 5h and disappeared at 24h after GIR. Faint Fos expression appeard in the CA1 and CA2 fields at 5h and disappeared at 32 -48h after GIR. The sequence of the strength and time course for Fos expression in the hippocampus was DG, CA3, CA4, CA2 and CA1. These characteristics of Fos expression might be involved in mediating the expression of such as late genes dynorphin and nerve growth factor following ischemia insults.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1994年第4期344-348,共5页
Chinese Journal of Anatomy
关键词
脑缺血
海马
免疫组织化学
cerebral ischemia
hippocampus
immunocytochemistry
