摘要
取ＳＤ大鼠２９只，制成大鼠局灶性脑缺血模型，观察再灌流０．５～４８小时时神经元的改变与巨噬细胞应答，并探讨两者间的相互关系。结果：再灌流３小时内，神经元主要表现为收缩或肿胀；再灌流６小时组，有散在的红神经元及鬼影细胞；再灌流４８小时组，缺血半影区有大量的凋亡细胞及凋亡小体。凝集素ＧＳＩ－Ｂ４标记巨噬细胞表明，１２小时时出现小胶质细胞活化及来自脑膜的巨噬细胞，２４小时时出现脑实质内血单核细胞浸润；４８小时时脑内巨噬细胞在缺血半影区大量聚集。上述结果表明：①脑缺血后阻止细胞凋亡和减少脑梗塞体积对脑梗塞患者有极重要的意义；②不可逆的神经元损伤早于小胶质细胞活化，来自脑膜的巨噬细胞早于脑实质血单核细胞浸润。该研究结果也为脑梗塞最佳药物治疗时间的选择提供了参考资料。
Male SD rats(n=29) were subjected to 2 hours' middle cerebral artery occlusion and were killed at various times of reperfusion(0.548 hours).The histological features of neuronal changes,the macrophagic response and the relationship between them were investigated. The neurons underwent acute changes(shrinking or swollen),and the pyknotic apoptotic neurons were occasionally seen within 3 hours. The number of apoptotic neurons increased with recirculation time. The scattered red neurons and ghost cells were first found at 6 hours of reperfusion. Many apoptotic neurons, apoptotic bodies were detected in the penumbra of ischemic core at 48 hours. Lectin histochemistry with GSIB4 was performed to observe the macrophage. The results showed the active microglia and macrophage from meninges presented at 12 hours. The macrophages from parenchyma vessels appeared at 24 hours. Reactive microglia and extrinsic macrophage aggregated into the band of macrophage which phagocytized the apoptotic Neuronal changesMacrophage responseneurons. The results indicate that, firstly, it is important to prevent the neuronal apoptosis and reduce the volume of infarct in stroke patients; secondly, irreversible neuronal lesions simultaneously stimulate microglial activation and the presence of extrinsic macrophages from meninges was earlier than the appearance of the macrophages from parenchyma bloodmonocytes. The authors also suggest that the data on the neuronal changes over time after ischemia and reperfusion be useful reference materials for selecting the most timely medication to treat cerebral infarction.
出处
《华西医科大学学报》
CSCD
1999年第2期155-157,137,共4页
Journal of West China University of Medical Sciences
