摘要
目的建立日本大耳白兔蛛网膜下腔出血（SAH）后症状性脑血管痉挛模型。方法结扎双侧颈动脉后枕大池二次注血方法制成兔SAH后症状性脑血管痉挛模型。采用随机数字表法将兔分为SAH组和对照组，每组12只。采用三维CT血管造影（3D—CTA）检测SAH前后基底动脉直径，并观察进食量、神经功能变化，5d后取海马分别行电镜观察与超氧化物歧化酶（SOD）、丙二醛（MDA）测定。结果与对照组比较，SAH组兔出现严重的神经功能障碍，5d后基底动脉出现明显痉挛，海马神经元出现核固缩，线粒体空泡样变等神经损伤的改变，SOD活性降低，MDA含量升高。结论该模型可以作为SAH后症状性脑血管痉挛的实验模型。
Objective To establish a rabbit model of symptomatic cerebral vasospasm after subarachnoid hemorrhage （SAH）. Methods The SAH model was established by ligation of bilateral carotid arteries and injection of autologous arterial blood into cistern magana twice in white Japanese rabbits. The diameters of basilar artery were measured by 3D-CTA before the operation （d0） and after the operation （d5）. The hippocampus was removed and observed by electron microscope, and the contents of superoxide dismutase （SOD） and malondialdehyde （MDA） in hippocampus were measured. Results Compared with the control group the neurological function was decreased in SAH group, severe basilar arterial vasospasm was found after d5. The neuron injury was observed with electron microscope in SAH group, including karyopycnosis and mitochondria vascuolated. Compared with the control group, SOD activity decreased and MDA increased in SAH group. Conclusion The established animal model of symptomatic cerebral vasospasm after subarachnoid hemorrhage can be used to evaluate the degree of secondary brain injury.
出处
《浙江医学》
CAS
2011年第11期1643-1646,共4页
Zhejiang Medical Journal
基金
浙江省科技计划项目（2008C33037）
关键词
脑血管痉挛
模型
动物
脑损伤
Vasospasm intracranial Models Animal Brain injuries
