摘要
目的探讨实验性阿尔茨海默病模型大鼠脑内海马光镜下的变化和DNA修复蛋白PCNAmRNA表达的变化,进一步探讨神经保护肽慢病毒rLent/NT4-NAP(NAP)对其具有神经保护作用机制。方法选择无菌级雄性大鼠Wister大鼠40只,随机分为对照组,假手术组,阿尔茨海默(AD)模型大鼠组,rLent/NT4-NAP(NAP)组,每组10只。建立Aβ1-40联合应用转移生长因子β1(TGFβ1)注入实验大鼠左侧海马区制备阿尔茨海默动物模型,通过HE染色观察各组大鼠海马区的形态学改变;通过原位杂交的方法检测实验各组大鼠海马区PCNAmRNA表达的变化。结果 HE染色发现痴呆组大鼠左侧海马区神经细胞明显变性、坏死,而NAP组细胞损伤情况较痴呆组大鼠明显减轻;用原位杂交的方法检测痴呆组大鼠左侧海马区神经细胞胞核内PCNAmRNA强阳性表达减少,而NAP组左侧海马区神经细胞胞核内PCNAmRNA表达较痴呆组显著上调与对照组比较无统计学差异。结论 NAP通过诱导DNA修复蛋白PCNA蛋白表达上调,从而促进DNA损伤修复和减少细胞凋亡,对神经细胞有神经保护作用,实现其内源性的保护作用。
Objective To reseach the change of PCNAmRNA and pathological on neuron protective effect of Lentivirus neuroprotective peptid to Alzheimer model rat.Methods In this expriment we make some rats as the Alzheimer model whose left hippocamp being injeced aggregated Aβ1-40 and transfer growth factor β1（TGFβ1）.These results tell us that NAP has neuroprotective function.Beta-amyloid protein injection into hippocampus in brain lissue were applied to make experimental rat model.The change of ethology and express PCNA-Mrna of hippocampal neurons in Alzheimer model rats after therapeutic lentivirus neuroprotective peptid in situ tecknique.Results NAP group on the change of in Alzheimer model rats the expressing PCNA-Mrna of hippocampal neurons were significant higher than those in AD group.Conclusion In this experiment,Proving the neuron protective effect of NAP to Alzheimer model.It is the foundation of the repair action on curing the Alzheimer disease.
出处
《中国实验诊断学》
北大核心
2011年第11期1807-1809,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅课题(200705160)
