摘要
目的:探讨表没食子儿茶素没食子酸酯(EGCG)对小鼠体内调节性T细胞(Treg)的产生及转化生长因子TGF-β1的表达和分泌的影响。方法:选择健康、雄性、体重相近的BALB/c小鼠40只,随机分为4组(n=10),设立不同剂量浓度的EGCG组(10、50、85 mg/kg)和对照组。EGCG组每天给予EGCG腹腔注射一次,连续注射3天,对照组每天给予等体积生理盐水腹腔注射一次,连续注射3天。采用流式细胞仪检测小鼠脾脏Treg细胞的产生。采用荧光定量PCR和ELISA方法分别检测小鼠脾脏细胞和外周血TGF-β1因子的表达和分泌水平。结果:与对照组相比较,EGCG处理组小鼠体内的Treg细胞的比例上升,TGF-β1的基因表达水平和蛋白分泌水平均明显增高,当EGCG剂量为85 mg/kg时,其促进作用最明显。结论:EGCG可促进小鼠体内的Treg细胞产生,增强TGF-β1表达和分泌。
Objective：To explore the effect of epigallocatechin-3-gallate（EGCG） on the population of regulatory T cells（Tregs） and the expression of transforming growth factor-β（TGF-β1） in vivo.Methods：The health male BALB/c mice were chosen and randomly divided into 4 groups（n=10）.Mice are treated with EGCG（10,50,85 mg/kg） for experiment group and Saline for control group by peritoneal injection once per day for three times.The effect of EGCG on the population of the splenic Treg cells was measured by FACS method.The levels of TGF-β1 mRNA in spleen were detected by Real time-PCR.The expression of TGF-β1 protein in peripheral blood was determined by Enzyme-Linked Immunosorbant Assay（ELISA）.Results：The population of Tregs in mice treated with EGCG was higher than those treated with Saline.In addition,EGCG could also increase the levels of TGF-β1 mRNA and the protein especially in an 85 mg/kg dose manner.Conclusion：EGCG can significantly promote the growth of Treg cells and the expression of TGF-β1 in vivo.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第11期974-977,共4页
Chinese Journal of Immunology
