摘要
以‘串枝红’ב金太阳’杏的F1代为材料,在分析共显性标记分离方式的基础上利用简单重复序列(SSR)和相关序列扩增多态性(SRAP)标记,构建了两张杏分子连锁图谱。‘串枝红’图谱共14个连锁群,包含37个SSR标记和32个SRAP标记,连锁群总长度为1086.6cM,每连锁群平均长度77.6cM,标记间平均连锁距离为19.7cM。‘金太阳’连锁图谱共7个连锁群,包括26个SSR标记和14个SRAP标记,连锁群总长度756.7cM,每个连锁群平均长度为108.1cM,标记间平均距离为22.9cM。分析了图谱构建过程中不同共显性标记在F1代分离类型中的利用。
Genetic linkage maps for two apricot cultivars have been constructed using simple sequence repea（tSSR）and sequence-related amplified polymorphism（SRAP）markers in 94 F1 individuals from the cross Chuanzhihong×Golden-sun.A total of 37 SSR and 32 SRAP markers were placed into 14 linkage groups on the Chuanzhihong map,defining 1 086.6 cM of the total map-length,and the average length of group was 77.6 cM.The average distance between adjacent markers was 19.7 cM.The male parent map of Golden-sun consisted of 26 SSR and 14 SRAP markers could be assigned to 7 linkage groups,defining 756.7 cM of the total map-distance.The average distance of group was 108.1 cM,with an average interval of 22.9 cM between adjacent markers.Segregation type of heterozygous loci in F1 was discussed in constructing map of apricot.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第10期1983-1990,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31071767)
公益性行业(农业)科研专项(201003058)
农业部作物种质资源保护项目(NB2010-2130135-03)
关键词
杏
杂合位点
分子标记
分离类型
连锁图谱
apricot
heterozygous loci
molecular markers
segregation type
linkage map
