摘要
对507对欲作人工流产的夫妇进行断面调查及绒毛组织微核的测定,以探讨父母吸烟、饮酒及采取避孕措施等因素对子代的致突变性。结果显示:微核的发生率与母亲年龄、孕次、孕龄、既往流产史等自身的因素无关;各种避孕措施及少量饮酒均不具有诱发绒毛微核细胞率增加的作用。然而,男方吸烟、女方被动吸烟其微核细胞率为0.7645±0.0561‰高于饮酒组(0.5667±0.2004‰)和非吸烟对照组(05522±0.0616‰),与对照组有显著性统计学意义(P<0.05)。男方配偶吸烟兼饮酒者其微核发生率最高(0.7944±0.0754‰),说明吸烟与饮酒对诱发绒毛微核的产生有协同作用的趋势。本文并对男方吸烟和/或女方被动吸烟可诱发胚胎绒毛组织细胞DNA和/或纺锤体装置的损伤,进行了探讨,提出应引起人们的高度重视,并建议利用人类绒毛组织微核测定法作为监测环境致突变因子对人类下一代影响的方法。
Rapid determination of DNA damage by micronucleus test is well accepted. Animal bone marrow cells or human peripheral lymphocytes used in most studies could nor directly reflect the influence of the mutagenic effect on the offsprings by environmental factors. Human cho-rionic villi micronucleus test to detect directly the mutagenic effect of environmental factors has not been reported in the literature. Direct determination of human chorionic villi micronuclei (CVMN) was established in our laboratory, to study the mutagenic effect of mother's age, gra-vidity, gestation age, abortion history, contraception (condom, diaphragm, rhythm, oral contraceptives, spermicide or IUD), smoking and drinking on the offsprings. Cross investigation and micronucleus test were used in 507 couples undergoing artificial abortion.Micronuclei were scored according to Countryman's standard. 2,000 interphases were observed in each subject for CVMN frequency (‰). Arcsine transformation {arcsine [Sqr (P)] } was used in transforming CVMN frequency and the analysis of variance were used for statistics.No correlation between CVMN frequency and mother's age, gravidity, gestation age, abortion history, and contraception was found.Neither smoking nor drinking habit was found among the women of this study. The CVMN frequency of husband smoking was 0.7645±0.0561‰, of husband non-smoking-drinking was 0.5522 ±0.0616‰, of husband drinking was 0.5667 ±0.2004‰, of husband smoking and drinking was 0.7944±0.0754‰. There was a statistical difference in CVMN frequency between husband smoking and non-smoking (F = 2.78 DF = 408 P<0.05). No significant difference was found between husband drinking and non-drinking.
关键词
绒毛
微核
人类
致突变性
Human, Chorionic villi, Micronucleus
