摘要
随机选择年龄２０～３０岁、孕６～９周拟行人工流产的健康孕妇６０名，其中３０名以Ｂ型超声显像诊断仪经下腹部持续照射子宫内孕囊１０分钟，２４小时后与其他未经照射的３０名孕妇均行人工流产术。取绒毛组织，以３２Ｐ标记Ａｌｕ探针点杂交法检测绒毛细胞单、双链ＤＮＡ。结果表明：照射组与对照组比较，绒毛细胞单、双链ＤＮＡ量及含量均无显著性差异（Ｐ＞０．０５）．说明本实验所用诊断超声强度和照射时间未引起绒毛细胞ＤＮＡ单、双链断裂。
Thirty normal pregnant women aged 20─30 who wanted to undergo induced abortion were randomly selected during 6─9 weeks of gestation. 24 hours after the exposure of uterus to diagnostic ultrasound,300─500 mg wet weight of chorionic villi were obtained during the abortion. 30 cases of similar status who did not receive ultrasound-exposure before curettage were selected as controls,and chorionic villi were collected druing curettage. Single-strand breaks and double-strand breaks in villus cells DNA in all cases were assayed primarily by using 32P-labeled Alu probe dot-bloting hybridization. No significant differences were noted between the two groups.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1995年第4期215-217,共3页
Chinese Journal of Medical Genetics
关键词
超声波诊断
B超
早期妊娠
绒毛
DNA
放射损伤
Early pregnancy Chorionic villus Ultrasound DNA Single-strand breaks Double-strand breaks
