摘要
利用蜗牛酶酶法对二醇型人参皂甙Rd进行转化,并制备稀有人参皂甙C-K.采用薄层色谱和高效液相色谱法,对转化产物及酶反应相关影响因素进行检测.结果发现:在酶量为166.7μkat.g-1,pH值为5.0,温度为40℃的恒温水浴9h的条件下,酶解人参皂甙Rd可得稀有人参皂甙C-K.在此基础上,研究得到酶反应的最适条件范围:酶量为500.1～833.5μkat.g-1,pH值为3～5,温度为40～50℃,反应时间为6～24h.
Rare ginsenoside C-K was prepared using enzymatic transformation with snailase from protopanaxadiol ginsenoside Rd. Products and influencing factors of enzymatic transformation were examined by TLC and HPLC. The results showed that rare ginsenoside C-K could be obtained at the following conditions, enzyme amount 166. 7 μkat · g^-1, pH 5.0, thermostatic water bath at 40 ℃ for 9 h. Based on that, the optimum ranges of enzyme amount, pH value, tempera- ture and reaction time were further determined as 500. 1-833. 5 μkat · g^-1, pH 3-5, 40-50℃, 6-24 h, respectively.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2011年第6期668-671,共4页
Journal of Huaqiao University(Natural Science)
基金
福建省高等学校新世纪优秀人才支持计划(2006年度)
