摘要
目的探讨hMOF(human male absent on the first)蛋白在子宫内膜癌组织中的表达及临床意义。方法采用免疫组织化学(IHC)的方法检测58例子宫内膜癌组织(子宫内膜癌WTO分级G1级20例,G2级18例,G3级20例)及癌旁正常组织的hMOF蛋白的表达水平,并与临床及病理资料进行分析比较,统计学方法采用单因素多元方差分析及Fisher检验明确hMOF蛋白表达水平与子宫内膜癌患者临床病例特点的关系。结果免疫组织化学显示:hMOF蛋白在23例(39.6%)子宫内膜癌组织中的表达为阴性或弱阳性,癌旁正常组织仅5例(13.1%)表达为阴性或弱阳性,两组有显著差异(P<0.05)。根据IHC评分将患者分为≤4分组和≥5分组,比较两组间患者的临床特点:≤4分组中,子宫内膜癌为低分化(G3级)的病例占52.1%(12/23),FIGO分级为Ⅲ-Ⅳ级的病例占47.8%(11/23);≥5分组中G3病例仅占22.8%(8/35),FIGO分级为Ⅲ-Ⅳ级的病例占14.3%,两组间差异显著(P<0.05)。结论 hMOF蛋白在子宫内膜癌组织,尤其低分化、高分期的子宫内膜癌组织中的表达明显下降。这提示hMOF蛋白可能在子宫内膜癌的发生发展中起到重要作用,hMOF蛋白可以作为一个判断子宫内膜癌疾病严重程度和预后的生物分子指标。
Objective To study Expression of hMOF protein in endometrial carcinoma and relationship of hMOF protein and its clinical characteristic.Methods To detect the expression of hMOF protein in 58 samples of endometrial carcinoma tissues（20 cases is G1,18 cases is G2 and 20 cases is G3） and its paracarcinoma tiusses by immunohistochemical SP method and to analyses its the clinical and pathological characteristic with Univariate analysis and Fisher test.Results Immunohistochemistry showed that： hMOF protein in 23 cases（39.6%） endometrial cancer tissues were negative or weakly positive,normal tissues only 5 cases（13.1%） were negative or weakly positive,the hMOF protein expression of the two groups was significant differences（P0.05）,According to IHC score all samples were divided into two groups： ≤ 4 group and ≥ 5 group,compared the clinical characteristics between the two groups： ≤ 4 groups,the endometrial cancer is poorly differentiated adenocarcinoma（G3） accounted for 52.1% of cases（12/23）,FIGO stage Ⅲ-Ⅳ classification of cases accounted for 47.8%（11/23）;≥ 5 groups of poorly differentiated adenocarcinoma（G3） cases accounted for only 22.8%（8 /35）,FIGO stage Ⅲ-Ⅳ classification of 14.3% of cases,significant difference between the two groups（P0.05）.Conclusion hMOF protein in endometrial cancer,especially high-grade and high stage endometrial cancer tissues was significantly decreased.This prompted hMOF protein may play an important role in the development of endometrial cancer,hMOF protein can serve as a biomolecular marker of disease severity and prognosis of endometrial cancer.
出处
《中国实验诊断学》
北大核心
2011年第10期1648-1651,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金资助项目(编号31070668)
