摘要
目的观察七叶皂苷钠对人胆管癌细胞株Hccc9810增殖和凋亡的影响。方法运用细胞染色、噻唑蓝（MTT）比色法、流式细胞术和实时荧光定量聚合酶链反应（PCR）等技术观察不同浓度（、10、20、40μmol／L）七叶皂苷钠对胆管癌细胞增殖、细胞周期和凋亡的影响。结果七叶皂苷钠呈时间剂量依赖性抑制胆管癌细胞Hccc9810的增殖，其24、48、72h的50％抑制浓度（IC50）分别为：（45．34±2．12）、（33．00±1．92）、（25．00±1．65）μmol／L；G1期比例从（49．49±1.22）％上升到（70．20±1．52）％；胆管癌细胞形态发生典型凋亡特征性改变，凋亡率随浓度的升高从20．54％上升到62．56％，差异均有统计学意义（P〈0．05）。结论七叶皂苷钠通过阻滞细胞周期和诱导细胞凋亡而抑制胆管癌细胞Hccc9810增殖。
Objective To investigate the effects of sodium aescinate on the proliferation and apoptosis of cholangiocarcinoma Hece9810 cell line. Methods The effects of sodium aeseinate （0,10, 20, 40μmol/L） on cell growth, cycle and apoptosis were investigated by methyl thiazol tetrazolium （MTT） assay, flow cytometry, and fluorescence microscopy. Results Sodium aescinate inhibited cholangiocareinoma cell growth in a dose- and time-dependent manner, and its half-inhibitory concentration （ IC50 ） for 24 h, 48 h, and 72 h was （45.34 ±2. 12）, （33.00 ± 1.92） and （25.00 ± 1.65） μmol/L respectively. The proportion of cells in G1 phase was increased from （49.49 ± 1.22） % to （70. 20 ±1.52） %. The apoptosis rate was increased from 20. 54% to 62. 56% （P 〈 0. 05 ）. Conclusion Sodium aeseinate exhibited its anticarcinoma effect probably through regulating cell cycle and inducing cell apoptosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第11期1835-1837,共3页
Chinese Journal of Experimental Surgery
关键词
七叶皂苷钠
增殖
胆管癌
Sodium aeseinate
Proliferation
Cholangioeareinoma
