摘要
目的建立能同时检测甘地胶囊样品中2种指标成分(黄芪甲苷、黄芩苷)含量的反相高效液相色谱法。方法样品用超声提取法,用Inertsil(?)ODS-3色谱柱分离样品,流动相为乙腈与0.5‰磷酸(pH 2.25),梯度洗脱,流量为1 mL·min^(-1),柱温为室温,检测波长215 nm;外标法定量。结果对于待测组分r均>0.999;黄芪甲苷的线性范围为0.5～50 g.L^(-1);黄芩苷的线性范围为0.01～50 mg·L^(-1);检出限为黄芪甲苷0.1 g·L^(-1),黄芩苷0.01 mg·L^(-1)。方法的加样回收率黄芪甲苷、黄芩苷为96.89%～100.00%,RSD低于3.5%(n=3)。结论本法简便、准确、灵敏度高,可用于甘地胶囊中多指标成分的含量测定,也可用于制剂指标成分的研究。
A/M To develope a reversed phase high performance liquid chromatography to determine multiple ingredients in Gandi capsule. METHODS Gandi capsule samples were extracted with selective ultrasound extraction. Separation and quantification were achieved by a Inertsil ODS - 3 column under the optimum conditions. Acetonitrile and 0.5%o phosphoric acid solution （ pH 2.25） were used as mobile phase and gradient elution at a flow rate of 1 mL· min^-1, at room temperature and detected with UV detector at 215 urn. RESULTS The quantified analysis of external standard calibration curves was used. The calibration graph was linear in the range of 0.50- 50 g·L^-1 for astragaloside（AG）and 0.05 - 50 mg·L^-1 for baicalin（BCL） with r 〉 0.999, respectively. Detection limit for AG was 0.1 g·L^-1 and for BCL was0.01 mg·L^-1. For spiked sample analysis, the RSD was less than 3.5% and the recoveries were 96.89% - 100.00% for AG and BCL. CONCLUSION This method is simple,accurate and convenient,and can be used to determine index components of Gandi capsule.
出处
《中国临床药学杂志》
CAS
2011年第5期296-299,共4页
Chinese Journal of Clinical Pharmacy
基金
上海交通大学医学院科技基金(编号JYY0905)
