摘要
背景:滑膜细胞信号转导异常是类风湿性关节炎的重要发病机制之一。中药复方痹肿消汤治疗类风湿性关节炎具有较好的临床疗效,但在细胞信号转导水平探讨痹肿消汤治疗类风湿性关节炎的作用机制是十分必要的。目的:观察中药复方痹肿消汤对胶原诱导关节炎模型大鼠滑膜细胞外信号调节激酶1/2,5表达的影响。方法:SD大鼠45只随机数字表法分为对照组、模型组、痹肿消汤组。胶原诱导关节炎模型大鼠,用痹肿消汤药液3.0～3.5mL灌胃,1次/d,连续14d。检测造模后第14,21,28天大鼠滑膜细胞外信号调节激酶1/2,5的磷酸化水平。结果与结论:造模后,大鼠滑膜细胞外信号调节激酶1/2,5的磷酸化表达随时间而增加(P<0.05)。痹肿消汤治疗后,细胞外信号调节激酶1/2,5磷酸化表达水平则明显下降(P<0.05)。提示痹肿消汤能抑制细胞外信号调节激酶1/2,5蛋白激酶的激活,从而抑制细胞外信号调节激酶1/2,5信号转导通路的活化,这可能是其阻抑类风湿性关节炎滑膜异常增殖、关节骨质侵蚀的重要机制之一。
BACKGROUND：Abnormal signal transduction of synovial cells is one of important pathogeneses of rheumatoid arthritis （RA）. Chinese herbs Bizhongxiao decoction exhibits satisfactory clinical curative effects in treatment of RA. It is necessary to investigate the mechanism by which Bizhongxiao decoction treats RA at the level of cellular signal transduction. OBJECTIVE：To investigate the effects of Bizhongxiao decoction on extracellular signal-regulated kinase （ERK） 1/2 and ERK 5 expression in the synovial tissue in a rat model of collagen-induced arthritis. METHODS：Forty-five SD rats were randomly divided into three groups：control, model and Bizhongxiao deconction. Rat models of collagen-induced arthritis were intragastrically administered with 3.0-3.5 mL Bizhongxiao decoction, once a day, for successive 14 days. At 14, 21 and 28 days after arthritis induction, ERK 1/2 and ERK 5 expression was detected. RESULTS AND CONCLUSION： After arthritis induction, ERK 1/2 and ERK 5 expression in the synovial tissue was increased with elongation of time （P 0.05）. After Bizhongxiao decoction treatment, ERK 1/2 and ERK 5 expression levels were significantly decdreased （P 0.05）.Results showed that Bizhongxiao decoction can inhibit the activation of ERK1/2, and ERK 5 and thereby inhibit the activation of ERK 1/2 and ERK 5 signal transduction pathway. This may be one of important mechanisms by which Bizhongxiao decoction inhibits abnormal proliferation of synovial tissue and the erosion of articular bone in RA.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第37期6967-6971,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家教育部高校博士点基金(200805330011)
湖南省科技计划项目(2009SK3163)
湖南省中医药基金(2010038)~~
