摘要
目的通过中药复方痹肿消汤对实验性关节炎大鼠滑膜annexinl、aldolase A表达影响的同步研究,探讨痹肿消汤治疗类风湿关节炎(rheumatoid arthritis．RA)的作用机制。方法采用皮下注射牛Ⅱ型胶原与完全福氏佐剂诱导SD大鼠实验性关节炎(collagen-induced arthritis,CIA)模型,并设立正常对照组。采用免疫印迹法(Western blot)同步检测正常组、模型组及痹肿消汤组大鼠在不同时间点滑膜annexinl、aldolase A的表达,运用ImageTool 3．0灰度扫描软件对结果进行半定量分析。结果造模25d后,模型组滑膜annexm 1表达低于正常组(P＜0．05),aldolase A表达高于正常组(P＜0．05)。随着免疫时间的延长,模型组滑膜annexin 1表达呈水平递减趋势(P＜0．05),aldolase A表达则逐渐递增(P＜0．05)。痹肿消汤治疗后,annexin 1表达在各时间点明显均高于模型组(P＜0．05),表达呈递增趋势(P＜0．05),aldolase A表达则明显低于同时间模型组(P＜0．05),在25、35和45d水平,表达呈递减趋势(P＜0．05)。BZXD能上调CIA大鼠滑膜annexin 1表达、下调aldolase A表达。结论在CIA病理过程中,随着关节炎症状加重,annexin 1表达下调,aldolase A表达上调。提示anncxin 1、aldolase A与RA滑膜病变(关节炎症、骨质侵蚀)密切相关；BZXD上调CIA大鼠滑膜annexin 1表达、下调aldolase A表达,这可能是其阻抑RA关节炎症、骨质侵蚀的重要机制之一。
Objective To elucidate the mechanism of bizhongxiao decoction (BZXD) in treatment of rheumatoid arthritis (RA) by analyzing the effects of BZXD on annexin 1 and aldolase A expressions in synovium of CIA rats.Methods The ex- periment arthritis rat model was established by subcutaneous injection ofⅡcollagen and complete Freunds adjuvant.Annexin 1 and aldolase A expressions at different times in three groups were detected by Western blotting,and semi - quantification analysis was performed with ImageTool 3.0 gray scale scanning software.Results Annexin I expression of synovium in model group on the 25^(th) day was lower than that in normal group(P< 0.05),and atdotase A expression was higher than that in normal group (P＜0.05).Annexin 1 expression of synovium was down- regulated step by step in model group (P＜0.05), and aldolase A expression was increased gradually (P＜0.05).After BZXD treatment,annexin 1 expression was significantly higher than that in model group at the different timepoints(P＜0.05) and increased gradually (P＜0.05 ),while aldolase A ex- pression was significantly lower than that in model group at the different timepoints (P＜0.05) and decreased gradually (P＜0.05).Conclusion During the process of CIA,with the increasing severity of arthritis,annexin 1 edged down and aldola- se A edged up.It is suggested that annexin 1 and aldolase A are related to CIA synovitis and RA.BZXD can edge up annexin 1 expression and edge down aldolase A expression in synovial tissue of CIA rat,which may be one of its therapeutic mecha- nisms for RA.
出处
《实用预防医学》
CAS
2006年第6期1401-1405,共5页
Practical Preventive Medicine
基金
国家自然科学基金资助项目(30371833)
