摘要
目的观察胶原诱导性关节炎大鼠肿瘤坏死因子-α(TNF-α)mRNA表达,从基因水平探讨TNF-αmRNA在类风湿性关节炎的作用。方法将22只大鼠随机分为正常组和模型组2组,皮下注射Ⅱ型胶原蛋白配合完全弗氏佐剂诱导关节炎模型,采用半定量RT—PCR法测定胶原诱导性关节炎大鼠滑膜细胞TNF-αmRNA的表达。结果TNF-αmRNA在正常组有少量表达；25、45d模型组TNF-αmRNA表达明显高于正常组(P＜0．05),45d模型组表达高于25d模型组(P＜0．05)。结论在胶原诱导性关节炎大鼠中TNF-αmRNA的表达随着时间的增加而增设,提示了TNF-α基因表达与关节症状轻重有关,是介导RA炎症发展的主要因素。
Objective To observe the effect and significance of tumor necrosis factor -α(TNF-α)mRNA of C-Ⅱin- duced arthritis(CIA) in rat synovium for exploring the pathogenesis in rheumatoid arthritis.Methods Twenty - two rats were randomly divided into 2 groups.The rat experimental model was established by subculaneous injection of typeⅡcollagen and complete Fraund' s adjuvant.The expression of TNF-αmRNA in synovium of typeⅡcollagen induced arthritis in rats were detected by the method of RT- PCR.Results TNF -αmRNA in the normal group expressed slightly.Expressions of TNF -αmRNA in the model group at 25d and 45d were both significantly higher than those of the normal group(P＜0.05),and ex- pression in the model group at 45d was higher than that of the model group at 25d(P＜0.05 ).Conclusion Expression of TNF-αmRNA in CIA indicates that TNF -αgene expressian is relevant with the syndrome of joints in RA and it is the main factor of development in RA.
出处
《实用预防医学》
CAS
2006年第6期1405-1407,共3页
Practical Preventive Medicine
基金
国家自然科学基金项目(30371833)
湖南省科技基金项目(02ssy4028)
湖南中医药基金项目(20053)
