摘要
目的了解经颅高压电烧伤对大鼠肠系膜微血管内白细胞流变行为的影响，以及乌司他丁（UTI）对其的干预作用。方法将45只SD大鼠按随机数字表法分为假伤组、电伤组和治疗组，每组15只。电伤组和治疗组大鼠均用凋压器及实验变压器制成高压电烧伤模型；假伤组大鼠接相同装置但不通电，致假伤。假伤组和电伤组伤后2min内腹腔注射生理盐水2mL，治疗组于伤后2min内按2×10^4U／kg腹腔注射UTI2mL。用布氏显微镜及微循环图像分析系统观测3组大鼠伤前15min及伤后5min、1h、2h、4h、8h肠系膜微血管内滚动白细胞数、白细胞滚动速度、白细胞黏附数、白细胞．内皮细胞接触时间（TLECT）。对实验数据行t检验。结果（1）微静脉滚动白细胞数：电伤组和治疗组大鼠伤后各时相点滚动白细胞数较伤前增多，伤后5min分别剧增至（51．4±3．2）、（24．6±1．9）个／min，多于假伤组的（1．1±0．7）个／min（t值分别为59．28、44．99，P值均小于0．05）；治疗组伤后各时相点滚动白细胞数均少于电伤组，伤后5min组间比较，t=27．97，P〈0．05。（2）微静脉白细胞滚动速度：电伤组和治疗组伤后各时相点白细胞滚动速度较伤前下降，伤后5min最慢，分别为（90±9）、（175±13）um／s，均慢于假伤组的（277±12）um／s（t值分别为47．97、21．59，P值均小于0．05）；治疗组伤后各时相点白细胞滚动速度均快于电伤组，伤后5min组间比较，t=20．55，P〈0．05。（3）微静脉白细胞黏附数：电伤组和治疗组伤后各时相点白细胞黏附数较伤前增多，伤后5min即达每100微米血管（23，27±3．20）、（5．80±1．61）个，均多于假伤组的每100微米血管0个（t值分别为28．16、13．95，P值均小于0．05）；治疗组伤后各时相点白细胞黏附数少于电伤组，伤后5min组间比较，t=18．89，P〈0．05。（4）微静脉TLECT：电伤组和治疗组伤后各时相点TLECT较伤前增高，伤后5min即达（14．45±1．99）、（3．66±0．96）s／min，均多于假伤组的0s／min（t值分别为28．12、14．77，P值均小于0．05），治疗组伤后各时相点TLECT低于电伤组，伤后5min组间比较，t=18．91，P〈0．05。（5）3组大鼠伤前及伤后微动脉、毛细血管血流中，均未见白细胞滚动或者贴壁黏附。结论经颅高压电烧伤可导致大鼠肠系膜做静脉白细胞流变行为异常，UTI对此有明显改善作用。
Objective To study the influence of transcranial high-vohage electrical burn （HEB） on rheologiea] changes of leukocytes in mesentery capillary in rats and the therapeutic effects of ulinastatin. Methods Forty-live SD rats were divided into control （C） , electrical burns （EB） , and ulinastatin treatment （UT） groups according to the random number table, with 15 rats in each group. Model of HEB was reproduced in rats of EB and UT groups with voltage regulator and experimental transformer, and then rats in EB group was intraperitoneally injected with 2 mL isotonic saline while rats in UT group was intraperitoneally injected with 2 mL ulinastatin （2 × 10^4 U/kg）. Rats in C group received sham burn with the same treatment as used in EB group but without electric current. Rheological changes of leukocytes in mesentery capillary were observed with Bradford microscope at 15 minutes before HEB and 5 minutes, 1, 2, 4, 8 hour （s） after HEB （PHM or PHH）, including counting the number of rolling leukocytes, leukocytes rolling speed, the number of leukocytes adherent to mesentery capillary, total leukocyte-endothelium contact time （ TLECT）. Data were processed with t test. Results （ 1 ） The number of roiling leukocytes from PHM 5 to PHH 8 was increased in EB group and UT group as compared with that at 15 minutes before HEB, especially at PHM 5 [ （51.4 ±3.2）, （24. 6 ± 1.9） cells/min, respectively] which were higher than that in C group [ （1.1 ± 0.7） cells/min, with t value respectively 59.28, 44.99, P values all below 0.05 ]. The number in UT group at each time point after burn was less than those in EB group, especially at PHM 5 （ t = 27.97, P 〈 0.05）. （2） Compared with that at 15 minutes before HEB, the rolling speed of leukocytes from PHM 5 to PHH 8 was slow in EB group and UT group, especially at PHM 5 [ （90±9）, （175 ± 13） um/s, respectivelyl which were slower than that in C group [ （277 ± 12） um/s, with ;value respectively 47.97, 21.59, P values all below 0.05 ]. The rolling speed in UT group from PHM 5 to PHH 8 was faster than that in EB group, especially at PHM 5 （ t =20.55, P 〈0.05）. （3） Compared with that at 15 minutes before HEB, the number of leukocytes per 100 micrometer capillary from PHM 5 to PHH 8 was increased in EB group and UT group, especially at PHM 5 （23.27 ±3.20, 5.80 ± 1.61, respectively） which were higher than that in C group （0, with t value respectively 28.16, 13.95, P values all below 0.05）. The number of adhered leukocytes in UT group at each time point after burn was less than that in EB group, especially at PHM 5 （ t = 18.89, P 〈 0.05）. （4） Compared with that at 15 minutes before HEB, TLECT from PHM 5 to PHH 8 was increased in EB group and UT group, especially at PHM 5 [ （ 14.45 ± 1.99） , （ 3.66 ± 0.96） s/min, respectively ] which were longer than that in C group （0 s/min, with t value respectively 28. 12, 14.77, P val- ues all below 0.05）. TLECT in UT group from PHM 5 to PHH 8 was shorter than that in EB group, especially at PHM 5 （ t = 18.91, P 〈0.05）. （5） No rolling leukocyte or wall-adherent leukocyte was found in blood flow of arterioles or capillaries of rats in three groups at each time point. Conclusions Transcranial HEB can lead to abnormal rheological changes of leukocytes in mesentery capillary in rats, and the changes can be ameliorated by ulinastatin.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2011年第5期375-380,共6页
Chinese Journal of Burns
基金
基金项目：河北省自然科学基金（302551）
关键词
烧伤
电
肠系膜
微循环
白细胞
血液流变学
乌司他丁
Burns, electric
Mesentery
Microcirculation
Leukocytes
Hemorheology
Ulinastatin
