摘要
目的研究化瘀理肺方对大鼠肺纤维化肺组织形态学的影响及可能作用机制。方法将大鼠随机分为对照组、模型组、中药组、激素组,经气管内注入博莱霉素(5 mg/kg)建立肺纤维化模型,对照组注入生理盐水0.25 ml作为正常对照,造模后第2 d开始分别给予化瘀理肺方药物灌胃(中药组,13410 mg/kg)、生理盐水灌胃(对照组、模型组)、氢化可的松琥珀酸钠腹腔注射(激素组,25 mg/kg)进行干预,后于给药后的14 d、28 d分别处死,取肺组织行HE染色观察病理变化情况,免疫组化法测定肺组织中Smad3蛋白的表达情况。结果中药组肺泡炎和纤维化程度明显低于模型组和激素组,中药组Smad3蛋白表达明显少于模型组和激素组(P<0.01)。结论化瘀理肺方对博莱霉素所致肺纤维化大鼠有一定预防性治疗作用,可能部分通过调节Smad3蛋白表达发挥作用。
Object To explore the protective effect and the possible mechanism of Huayulifei on pulmonary fibrosis in rats.Method The rats were randomly divided into control group,model group,Huayulifei group and hydrocortisone group.The rats were then intratracheally dripped with bleomycin（5 mg/kg） in experimental groups,while 0.9% saline in control group.Treatments started from the next day with a daily oral Huayulifei（13410 mg/kg） in Huayulifei group,intraperitoneal Hydrocortisone sodium succinate（25 mg/kg） in Hydrocortisone group,and oral saline in control group and model group,respectively.Rats were killed at 14 and 28 days after bleomycin,lungs isolated for Hematoxylin and eosinηstaining,and the expression of Smad3 protein was measured by immunohistochemical technique.Results The alveolitis and pulmonary fibrosis in Huayulifei group was alleviated significantly compared with those in model group and hydrocortisone group.The expression of Smad3 protein was significantly higher in model group and hydrocortisone group.Conclusion Huayulifei can ameliorate bleomycin-induced pulmonary fibrosis in rats;It may partly work by modulating the expression of Smad3 in lung tissue.
出处
《临床肺科杂志》
2011年第11期1675-1677,共3页
Journal of Clinical Pulmonary Medicine
