中药升陷汤抑制肺癌A549细胞增殖和侵袭转移作用研究被引量：18

Study for effect of Shengxiantang on invasion and metastasis of lung cancer A549 cell in vitro

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摘要目的:本研究旨在体外筛选出升陷汤抑制人肺癌A549细胞侵袭转移的有效萃取部位,并对其分子机制进行探讨。方法:通过MTT法和形态观察筛选出升陷汤抑制A549细胞增殖的有效萃取部位。Annexin V/PI双染法检测升陷汤正丁醇提取物对A549细胞的凋亡诱导作用。划痕实验和Transwell小室检测生陷汤提取物对A549细胞侵袭、转移的影响;Western blot检测侵袭转移相关信号通路分子表达的变化。结果:20-400μg/mL正丁醇萃取部位作用于A549细胞48、72h后可明显抑制其增长,且与凋亡相关。20-80μg/mL升陷汤作用于A549细胞24h后可明显抑制其侵袭和转移;正丁醇萃取部位可明显降低p-ERK和基质金属蛋白酶MMP2、MMP9的表达(P<0.01)。结论:升陷汤正丁醇萃取部位能显著抑制A549细胞的增殖和侵袭转移。 Objective： The aim of this study was to investigate the inhibitory effects of Shengxiantang（SXT） extract on A549 cells proliferation and explored its probable molecular mechanisms on inhibition invasion and metastasis.Methods： MTT assay was used to screen the effective component of SXT on proliferation of A549 cells.Cells apoptotic rate was investigated using Annexin V/PI staining and flow cytometer.Scratch and transwell assays were performed to measure the effects of shengxiantang（20-80μg/mL） on invasion and metastasis of A549 cells.Protein expression was determined by Western blot.Results： 20-400μg/mL n-butanol extract could inhibit the growth of lung cancer A549 cell significantly after treatment for 48 and 72h,the mechanism of which was related with induction apoptosis.The expression of MMP2,MMP9 and p-ERK protein was involved in inhibiting invasion and metastasis of A549 cells（P〈0.01）.Conclusion： The n-butanol extract of SXT could inhibit the growth,invasion and metastasis of lung cancer A549 cells via down-regulated ERK signal pathway.

作者赵连梅孙佳玮颜晰王磊霍炳杰刘亚贤单保恩

机构地区河北医科大学第四医院河北医大学第一医院

出处《中华中医药杂志》 CAS CSCD 北大核心 2011年第9期2147-2150,共4页 China Journal of Traditional Chinese Medicine and Pharmacy

基金河北省中医药管理局资助项目(No.2009137)~~

关键词升陷汤 A549细胞凋亡侵袭转移 Shengxiantang A549 cells Apoptosis Invasion Metastasis

分类号 R285 [医药卫生—中药学]

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