摘要
为了研究Zfy基因在生精过程中的作用,采用RNA干涉的方法抑制Zfy基因的表达,设计并构建Zfy基因的siRNA重组表达载体(pSilencer5.1/Zfy225及pSilencer5.1/Zfy2122)。选取32只雄鼠随机分成4组,其中2组分别注射本研究构建的2个重组表达载体作为试验组,另2组分别注射相同体积空载体和生理盐水作为对照组,采用睾丸局部注射的方式导入雄鼠体内,间隔10d注射1次,共4次,最后1次注射17d后,采集睾丸组织,利用荧光实时定量PCR法检测Zfy基因mRNA的表达水平。结果表明,pSilencer5.1/Zfy2122干扰后Zfy基因的mR-NA表达水平显著降低,与对照组比较差异极显著(P<0.01);pSilencer5.1/Zfy225差异显著(P<0.05)。其结果可为研究Zfy基因在小鼠生精过程中的功能奠定基础。
This paper utilized RNAi inhibition the expression of Zfy gene. The recombination expression vectors pSilencer5.1/ Zfy225 and pSilencer5.1/Zfy2122 were constructed. 32 male Kunming Mus were divided into four groups randomly and evenly. The two recombination expression vectors were respectively injected into two groups through testis. The other two groups were injected with the same volume of physiological saline and empty vector pSilencerS, l-H1 Retro,respectively. They were injected every ten days and four times in all. 17 d after the fourth injection,the testis tissue of each group was collected,and the expres- sion level of Zfy mRNA was determined by fluorescence quantitation real time PCR （qRT-PCR）. The result showed that the expression of Zfy mRNA decreased significantly after injection of pSilencer5. 1/Zfy2122 （P〈0. 01）. In pSilencer5.1/Zfy225 group,the expression of Zfy mRNA was significantly lower than in control （P〈0. 05）. This research was the basis for study the function of Zfy in gonepoiesis.
出处
《石河子大学学报（自然科学版）》
CAS
2011年第4期448-451,共4页
Journal of Shihezi University(Natural Science)
基金
石河子大学研究生创新基金项目(YJCX2010-Y02)
