摘要
采用硅胶柱色谱和半制备反相高效液相色谱分离方法,对南海海洋放线菌Streptomyces lusitanus SCSIOLR32的次级代谢产物进行了研究,分离得到四个酰胺类化合物,经MS1、H和13 C NMR波谱分析鉴定为二甲基甲苯2,4-二氨基甲酸甲酯(1),甲苯2,4-二氨基甲酸乙酯(2),甲苯2,6-二氨基甲酸甲酯(3)和甲苯2,6-二氨基甲酸乙酯(4)。运用X-单晶衍射确定了1的结构式。其中化合物2和4是首次从自然界中分离得到。采用16S分子生物学方法鉴定该菌株为链霉菌属放线菌。
Four aromatic amide metabolites, toluene-2,4-dicarbamic acid dimethyl ester （ 1 ）, toluene-2,4-dicarbamic acid diethyl ester （2）, toluene-2,6-dicarbamic acid dimethyl ester （3） and toluene-2,6-dicarbamic acid diethyl ester （4） were isolated from the marine-derived actinomycetes Streptomyces lusitanus SCSIO LR32 from theSouth China Sea with silica gel column chromatography and revered phase semipreparative-HPLC. Their structures were identified by MS, 1H and 13 C NMR spectral data analyses. The structure of 1 was confirmed by single crystal X-ray diffraction. Compounds 2 and 4 were obtained from natural sources for the first time. The strain was .identified as a member of Streptomyces genus by analysis of its 16S rRNA gene sequence.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第4期591-595,共5页
Natural Product Research and Development
基金
中国科学院知识创新工程重要方向项目(KSCX2-YW-G-065
KZCX2-YW-JC202
KSCX2-YW-G-073
KZCX2-EW-G-12)
中国科学院南海海洋所领域前沿项目(LYQY200805)
关键词
海洋放线菌
链霉菌属
酰胺
次级代谢产物
marine actinomycetes
Streptomyces lusitanus
aromatic amide
secondary metabolites
