摘要
目的观察Twist蛋白、血管内皮生长因子（VEGF）mRNA及蛋白在喉鳞癌组织中的表达及其与临床病理特征的关系，并探讨其在喉鳞癌发生、发展中的作用及临床应用价值。方法应用原位分子杂交检测VEGFmRNA和免疫组织化学S-P法检测Twist蛋白、VEGF蛋白分别在80例喉鳞癌、30例癌旁组织中的表达。结果Twist蛋白、VEGFmRNA及蛋白与TNM分期、T分期、甲状软骨累及、淋巴结转移、远处转移有关（P〈0．05或〈0．01），Twist蛋白和VEGF蛋白与分化程度有关（P〈0．01或〈0．05），但VEGFmRNA表达则与分化程度无关（P〉0．05）。Twist蛋白与VEGF蛋白在喉鳞癌组织中的表达呈正相关（r=0．334，P〈0．01）；VEGF蛋白与VEGFmRNA在喉鳞癌组织中的表达呈正相关（r=0．484，P〈0．01）。结论Twist蛋白和VEGFmRNA及蛋白的过度表达对喉鳞癌发生、发展可能起协同作用。联合检测Twist蛋白和VEGFmRNA及蛋白对预测喉鳞癌浸润转移有重要意义，并可作为判断喉鳞癌生物学行为的指标。
Objective To observe the expression of Twist protein, vascular endothelial growth factor （VEGF） mRNA and protein in laryngeal squamous cell carcinoma （LSCC） and their relation to clinical pathological characteristics, and aslo debate their effect and clinical application in carcinogenesis, developing of LSCC. Method Molecular hybridization in situ was carried to detect VEGF mRNA, and immunohistochemistry S-P method was carried to detect the expression of Twist protein and VEGF protein in 80 cases of LSCC, 30 cases of paraneoplastic tissue. Results The expression of Twist protein, VEGF mRNA and protein was related to TNM stage, T stage, thyroid cartilage involvement, lymph node metastasis and distance metastasis （P 〈 0.05 or 〈 0.01 ）. The expression of Twist protein and VEGF protein was related to differentiated degree （P 〈 0.01 or 〈 0.05）, however, the expression of VEGF mRNA was not related to differentiated degree （P 〉 0.05 ）. The expression of Twist protein was positively related to the expression of VEGF protein in LSCC （r = 0.334,P〈 0.01 ） ; and the expression of VEGF mRNA was positive correlation to the expression of VEGF protein （r = 0.484,P 〈 0.01 ）. Conclusions Overexpression of Twist protein and VEGF mRNA and protein might play a joint action in the carcinogenesis, development of LSCC. Combined detection of Twist protein and VEGF mRNA and protein has vital significance in predicting the invasion and metastasis of LSCC, which might be an index to judge the biological behavior of LSCC.
出处
《中国医师进修杂志》
2011年第24期30-33,共4页
Chinese Journal of Postgraduates of Medicine
关键词
TWIST转录因子
血管内皮生长因子
喉肿瘤
免疫组织化学
Twist transcription factor
Vascular endothelial growth factor
Laryngeal neoplasms
Immunohistochemistry
