摘要
水稻（辽盐9）种子在LS＋2，4－D（2，4-二氯苯氧乙酸）2．0～3．0mg/L的培养基上进行诱导培养，经3～4次继代后，即可得到淡黄色颗粒性愈伤组织；将该种愈伤组织在LS＋2，4－D2．0～3．0mg／L＋LH（水解乳蛋白）300mg/L的液体培养基上进行振荡培养，经4～5次继代后，即可建立起良好的细胞悬浮系；培养基中附加2．0～2．5mg／LKT，使愈伤组织和悬浮细胞均可再生，其再生率分别为15．9％～17．9％和18．1％～20．8％。
The rice seeds(Liao Yan 9)were cultured and induced in the LS medium.The results showed that the medium of LS+2,4-D2.0～3.0mg/L could induce growth of callus. After 3～4times subculture,the callus induced was light yellow, grainy and available for suspension culture. The calli were inoculated into the medium of LS+2,4-D2.0～3.0 mg/L+LH300 mg/L and cultured on the rotatory shaker. After 4～5 times subculture, the suspension cells could be obtained. KT was suitable for plants regeneration of rice, and the concentration of it was about 2.0～2.5mg/L. The regeneration frequence of calli and suspension cells was 15.9%～17.9% and 18. 1%～20. 8% respectly.
出处
《河北大学学报（自然科学版）》
CAS
1999年第4期363-365,共3页
Journal of Hebei University(Natural Science Edition)
