摘要
将从棉株体内分离获得的内生菌Ala、73a接入棉花黄萎病菌菌株JC1B(落叶型)、BP2(非落叶型)的Czapek培养液中,培养不同时间后提取粗毒素,用考马氏亮兰G250法测定浓度,结果表明:对BP2产毒素抑制最强的为Ala菌体,抑制率达5197%;对JC1B产毒素能力抑制最强的是73a菌体,抑制率为7260%。同时,Ala、73a的代谢物对JC1B、BP2的产毒素和菌丝生长亦有明显的抑制作用。加入内生菌或代谢物后,其黄萎病菌毒素粗提液对棉苗致萎度下降。内生菌株处理棉种对出芽有促进作用,其中73a菌株的促进作用略强。
Endophytic bacteria Ala and73 a isolated from cotton tissues were inoculated into Czapek medium in which defoliating strain JC1 B and non defoliating strain BP2 were cultured.Crude toxin was extracted and determined. Inhibition of Ala to toxin production of BP2 was the strongestwith the inhibition rate of5 1 97%,while to JC1 B,73 a had the strongestinhibition with the rate of72 6%.At the same time,inhibition ofthe metabolic productof Ala and 73 a to toxin production and hypha growth of JC1 B and BP2 was obvious.Wilted degree ofcotton seedling caused by crude extraction oftoxin of Verticillium dahliaebecame weaker after endophytic bacteria or their metabolic products being added.Treating with endophytic bacteria could promote cotton seed germination,especially with73 a.
出处
《江苏农业学报》
CSCD
北大核心
1999年第4期211-215,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省重点攻关课题!BE993 14
江苏省应用基础项目!BJ980 2 4
