摘要
本研究在分离植物组织内生菌及根围土壤细菌的基础上,进行了室内拮抗活性测定,进而进行诱抗效果的检测。来自植物组织内部的细菌群落中含拮抗性较强的细菌群体较大。在拮抗群体中,经诱导,68.42%菌株获得了抗 Rif 300×10^(-6)的突变体。抗利福早突变体菌株在培养性状、室内拮抗性及诱导抗性等方面,与原菌株均十分相似。以菌株73a 诱导抗性效果较好,达67.21%。诱抗效果与细菌在体内定殖与运转能力密切相关。
This study focused on the induced resistance against Verticillium dahliae by pre- inoculation of bacterial strains that isolated from cotton tissues and rhizosphere soils. The results showed that the percentage of strong antibiotic strains from cotton tissues to total strains,6.80%,was higher than that from rhizosphere soils, 1.40%.Of 19 antibiotic strains isolated from cotton tissues,13 strains acquired Rif resistant mutants that showed the same cultural characteristics and antibiotic on petri dishes and induced resistance against V.dahliae as their original wild strains. The strains 73a from cotton tissues gave the greatest induced resistance, 67.21%,among 19 tested strains.The transferability of bacteria in Cotton vascular tissue possitively correspond to the induced resistance of bacteria against V.dahliae.
出处
《中国生物防治》
CSCD
1996年第1期7-10,共4页
Chinese Journal of Biological Control
基金
国家自然科学基金(39370478)。
关键词
棉花
大丽轮枝菌
内生细菌
根际细菌
诱导抗性
endophytic bacteria
rhizobacteria
cotton
Verticillium dahliae induced resistance
