摘要
研究了细胞分裂素在玉米愈伤组织诱导和植株再生中的作用，结果表明低浓度（０ ．２ ｍｇ／Ｌ） 的细胞分裂素能促进玉米幼胚诱导的愈伤组织再生，６ＢＡ 的效果比ＫＴ更好。不同品种的玉米幼胚诱导的愈伤组织的再生能力差异显著，普甜１ 号和苏玉１ 号的再生频率高达７８ ％ 和７５ ％ ，糯玉米和掖单９ 号仅为１０ ％ 和８ ％ 。植株再生途径也有所不同，普甜１ 号以器官发生为主要途径，苏玉１号则以体胚发生途径为主。经长期继代的愈伤组织失去再生能力，通过ＲＡＰＤ 方法比较发现胚性与非胚性愈伤组织的基因组之间存在差异，说明组织培养过程中愈伤组织的ＤＮＡ 发生了变异。
Cytokinin KT and 6 BA were evaluated for their function in callus induction and plant regeneration. The results showed that cytokinins improved the regeneration capacity of maize and 6 BA was more effective than KT. Five maize (Zea mays L.) varieties of commercial importance were screened for the regeneration capacity of calluses formed from their immature embryo. The difference in the rate of regeneration between 78% of Putian No.1 and 8% of Yedan No.9 were pronounced(Table 2). The mode of plant regeneration from type I callus also varied with the genotypes. Suyu No.1 tended to regenerate via somatic embryogenesis, while Putian No.1 was likely to regenerate via organogenesis. Maize callus lost the capacity of embryogenesis during long time subculture. The results of randomly amplified polymorphic DNA (RAPD) showed that its genome DNA was modified in tissue culture(Fig.6).
