摘要
以5个玉米品系幼胚为外植体，研究了基因型、2，4-D浓度以及胚龄对愈伤组织诱导的影响；6-BA对愈伤组织分化的影响；以及IBA对再生芽生根的影响。结果表明：除。31外，其他基因型的外植体在相同条件下均可诱导出愈伤组织，但是不同基因型间存在显著差异；2，4-D浓度和胚龄显著影响愈伤组织的诱导，且2，4-D浓度为2．0mg／L，胚龄在11—13d之间时，玉米愈伤组织诱导率较高且质量较好。将愈伤组织转入分化培养基后，6-BA促进了愈伤组织的再分化；在生根培养基中，IBA促进了再生芽生根，经过炼苗后移栽获得再生植株。
The immature embryo＇of five maize lines （Zea mays. L） was used to study the influencing of genotypes, 2,4- D concentration and em- bryo - age on callus induction, influencing of 6 - BA on callus differentiation , and influencing of IBA on buds rooting. The results showed that, callis were obtained fi＇om Q31 × Z31, Q31 × Z3,1373,Z3 except Q31. In addition, there were dominant differences among the induction percentage of four genotypes on the same conditions. The concentration of 2,4 - D in mediums and embryo- age had evident effects on the callus induction percentage of immature embryo. Adding 2.0mg/L 2,4- D in N6 medium and the embryo- age with lid to 13d were optimal for improving the percentage of inducing callus, The 6 - BA promoted callus differentiation and IBA promoted the buds rooting.
出处
《生物技术》
CAS
CSCD
2008年第4期62-64,共3页
Biotechnology
基金
国家科技支撑计划项目[2007BAD59B06]
贵州省科技厅转基因专项[黔科合2004NZ004]资助
关键词
玉米
幼胚
愈伤组织
诱导
植株再生
maize
immature embryo
callus
induction
plant regeneration
