摘要
采用硅胶柱层析法分离纯化产朊假丝酵母Y2.12发酵液,在考察洗脱剂种类、洗脱剂配比、上样液流速以及上样量这些单因素对叶酸分离效果的影响的基础上,通过响应面分析找出最优工艺参数。结果表明:产朊假丝酵母Y2.12发酵液分离纯化得到叶酸的最佳工艺条件为:上样液流速为1.26mL/min,发酵液上样量为5.29mL,洗脱剂乙醇与氨水之比1.84:1,所提取的叶酸质量为(101.12±0.13)μg。
Fermented broth of Candida utilis Y2.12 was separated and purified by silica gel column chromatography.The elution effects with different kinds of eluents,proportion of two eluents,flow rate and loading amount were studied.Based on single factor experiments,the proportion of ethanol and ammonia,flow rate,loading amount were analyzed by response surface methodology.The results showed that the optimal separation technological parameters were as follows：1.26mL/min flow rate,5.29mL loading amount,1.84：1 the proportion of ethanol and ammonia.The weight of extracted folic acid was（101.12±0.13）μg.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第8期304-306,309,共4页
Science and Technology of Food Industry
基金
国家教外司留([2001]660)
芜湖市科技计划项目(2008507)
安徽工程大学重点青年基金项目(2007YQ006zd)
关键词
硅胶柱
叶酸
分离
产朊假丝酵母
响应面分析
silica gel column
folic acid
separation
Candida utilis
response surface methodology
