摘要
目的:研究胰蛋白酶对IL-8释放的影响。方法:分离、培养人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)、倒置显微镜观察形态变化,流式细胞术检测内皮细胞标志和蛋白酶活化受体-2(proteinase-activated receptor-2,PAR-2)表达,ELISA检测HUVECs培养上清中IL-8水平。结果:HUVECs表达内皮细胞标志和PAR-2。刺激16 h,1 g/ml胰蛋白酶和100M PAR-2激活肽组HUVECs单层均匀性降低。胰蛋白酶能够显著刺激HUVECs释放IL-8,PAR-2激活肽也诱导IL-8水平升高。蛋白酶抑制剂和PAR-2抑制肽均能够显著抑制胰蛋白酶诱导的IL-8释放。PAR-2激活肽和胰蛋白酶诱导升高的IL-8水平之间成正相关性。结论:胰蛋白酶很可能通过PAR-2激活促进血管内皮细胞释放IL-8。
Objective： To investigate the effects of trypsin on interleukin-8 release. Methods： Human umbilical vein endothelial cells （HUVECs） were solated, cultured, and the monolayers in apperance were observed by inverted microscope. Expression of endothelial markers and proteinase-activated receptor-2（PAR-2） was detected by flow cytometry, and the IL-8 levels in culture supemants from HUVECs by ELISA. Results： There was the expression of endothelial markers and proteinase-activated receptor-2 （PAR-2）. The uniformity ofmonolayers decreased in HUVECs treated with 1 g/ml trypsin and 100 M PAR-2 peptide. Trypsin was able to up-regulate IL-8 release. A PAR-2 agonist peptide also significantly enhanced IL-8 release. Trypsin-induced IL-8 release was inhibited by proteinase inhibitors, and an inhibitor peptide. There was positive correlation between the IL-8 levels induced by trypsin and PAR-2 agonist peptide. Conclusion： Trypsin promotes IL-8 release most likely through PAR-2 activation.
出处
《现代生物医学进展》
CAS
2011年第15期2818-2821,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金(30471601
30570813)
关键词
内皮细胞
胰蛋白酶
蛋白酶活化受体-2
白细胞介素-8
Endothelial cells
Trypsin
Proteinase activated receptor 2
Inflammatory mediator
Interleukin-8
