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类胰蛋白酶对微血管内皮细胞中IL-6和TNF-α表达的影响 被引量：2

Effect of tryptase on the expression of IL-6 and TNF-α in microvascular endothelial cell
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摘要 目的研究肥大细胞类胰蛋白酶(tryptase)对小鼠脑微血管内皮细胞系bEnd.3表达白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的影响以及可能的信号途径。方法bEnd.3细胞与不同浓度的类胰蛋白酶共同培养后,用RT-PCR和放射免疫的方法检测细胞及其上清液中IL-6、TNF-αmRNA和蛋白的表达量以及蛋白酶激活受体2(PAR-2)对其表达的影响。结果类胰蛋白酶上调bEnd.3IL-6和TNF-αmRNA的表达和分泌,并呈时间和剂量依赖性,这一作用可能是通过激活细胞膜上的PAR-2而发挥的。结论肥大细胞类胰蛋白酶参与bEnd.3微血管内皮细胞的致炎性反应。 Purpose To study the effect of mast cell tryptase on the expression of IL-6 and TNF-α in bend. 3 （a mouse brain microvascular endothelial cell strain）. Methods bend. 3 cell strains were cultured with different concentrated tryptase. After isolating total RNA, reverse transcription polymerase chain reaction （RT-PCR） was made to examine the mRNA level of IL-6 and TNF-α in cells. Meanwhile,tryptase-induced cytokine secretion in the cultured supernatants was measured by radioimmunoassay. The effect of PAR-2 on the expression of IL-6 and TNF-α in bend. 3 was also explored. Results Tryptase induced up-regulation of the expression of IL-6, TNF-α mRNA and protein in concentration-dependent and time-dependent manners by activating protease-activated receptor 2 （PAR-2）. Conclusions Mast cell tryptase may be involved in pro-inflammatory reaction in microvascular endothelial cell.
作者 买买提祖农.买苏尔 马永杰 陆超 赵凤娣 殷莲华
机构地区 新疆医科大学病理生理学教研室 复旦大学上海医学院生理与病理生理学系
出处 《复旦学报（医学版）》 CAS CSCD 北大核心 2006年第2期217-221,共5页 Fudan University Journal of Medical Sciences
基金 国家自然科学基金(30470689)资助
关键词 类胰蛋白酶 蛋白酶激活受体2 白细胞介素-6 肿瘤坏死因子-α 炎症 tryptase proteinase activated receptor-2 interleukin-6 tumor necrosis factor-alpha inflammation
分类号 R363 [医药卫生—病理学]
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参考文献20

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共引文献20

同被引文献15

  • 1陆超,马永杰,买买提祖农.买苏尔,赵凤娣,殷莲华.类胰蛋白酶对血管内皮细胞表达干细胞因子的作用及机制[J].复旦学报（医学版）,2006,33(3):291-294. 被引量：1
  • 2Cecilia P. Chung,Ingrid Avalos,Paolo Raggi,C. Michael Stein.Atherosclerosis and inflammation: insights from rheumatoid arthritis[J].Clinical Rheumatology.2007(8)
  • 3Kinoshita, M,Okada, M,Hara, M,Furukawa, Y,Matsumori A.Mast cell tryptase in mast cell granules en-hances MCP-1 and IL-8 production in human endothelialcells[].Arteriosclerosis.2005
  • 4Malamud, V,Vaaknin, A,Abramsky, O,Mor, M,Burgess, L.E,Ben-Yehudah, A,Lorberboum-Golski, H.Tryptase activates peripheral blood mononuclear cells causing the synthesis and release of TNF-α, IL-6 and IL-1β: possible relevance to multiple sclerosis[].Journal de Neuroradiologie.2003
  • 5Monaco, C,Andreakos, E,Kiriakidis, S,Mauri, C,Bicknell, C,Foxwell, B,Cheshire, N,Paleolog, E,Feldmann, M.Canonical pathway of nuclear factor κB activation selectively regulates proinflammatory and prothrombotic responses in human atherosclerosis[].Proceedings of the National Academy of Sciences of the United States of America.2004
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  • 7Chung, C.P,Avalos, I,Raggi, P,Stein, C.M.Atherosclerosis and inflammation: insights from rheu-matoid arthritis[].Clinical Rheumatology.2007
  • 8Compton, S.J,Cairns, J.A,Holgate, S.T,Walls, A.F.The role of mast cell tryptase in regulating endothelial cell proliferation, cytokine release, and adhesion mole-cule expression: tryptase induces expression of mRNA for IL-1β and IL-8 and stimulates the selective release of IL-8 from human umbilical vei[].The Journal of Immunology.1998
  • 9Eickelberg, O,Roth, M,Block, L.H.Effects of am-lodipine on gene expression and extracellular matrix formation in human vascular smooth muscle cells and fibroblasts: implication for vascular protection[].Interna-tional Journal of Cardiology.1997
  • 10Gibbons,G.H.Endothelial function as a determinant ofvascular function and structure: a new therapeutic target[].The American Journal of Cardiology.1997

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二级引证文献9

复旦学报（医学版）
2006年 第2期

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