摘要
目的:对产自浙江省的11种铁线莲属药用植物的茎、叶中总黄酮含量进行测定分析,试图发现铁线莲属植物地上部分总黄酮含量较高的具体种类及铁线莲属植株中总黄酮含量较高的部位。方法:以芦丁为标品,采用紫外分光光度法。结果:(1)芦丁对照品在15.936～47.808μg·mL-1(r=0.9999)线形关系良好,平均回收率为100.8%,RSD=1.74%。(2)总黄酮含量总体趋势为叶>茎,铁线莲属植物叶中总黄酮平均含量为2.636%,茎中总黄酮平均含量为0.617%。(3)总黄酮含量最高的为女萎叶,达5.581%,最低为湖州铁线莲茎,仅0.195%。(4)铁线莲属植物不同物种的总黄酮含量差异显著,同一物种不同部位间的总黄酮含量也存在一定的差异。(5)聚类分析表明,铁线莲属地上部分总黄酮含量和该属植物系统演化有一定的相关性。结论:研究表明女萎、山木通地上部分较适合用作黄酮类药物开发,该研究同时将为铁线莲属不同药用种类、不同药用部位及药用植物亲缘关系的深入研究提供参考依据。
Objective： To find out the species and organs with higher content of total flavonoids in the aerial parts of 11 Clematis species collected from Zhejiang province. Methods： The ultraviolet spectrophotometric method was uesd with rutin as standard. Results： （1）There was good linearity （r = 0. 999 9 ）during the range of 15. 936 ～ 47. 808μg. mL-1 for rutin. The average recovery was 100. 8 % and the RSD = 1.74 %. （2）The total flavonoids from different parts of Clematis plants were different. The leaves contained more flavone than the stems, respectively the average content of total flavonoids in leaves and stems was 2. 636 % ,0. 617 %. （3）Clematis. apiifolia leaves had the highest total flavonoids,5. 581%, significantly higher than others while C. huchouensis only 0. 195 %. （4）Analysis showed that the amount of flavone in various plants and organs was significantly different. （5） Cluster analysis showed that there was a certain correlation between content of total flavonoids in the aerial parts of 11 Clematis species and evolution of this genus. Conclusion： This study suggested that the aerial parts of C. apiifolia and C. finetiana could be used as drug development of total flavonoids. And this study provided reference for further research of different species, different parts and phylogenetic correlation from medical Clematis resources.
出处
《中国现代中药》
CAS
2011年第7期14-18,共5页
Modern Chinese Medicine
基金
浙江省重大科技攻关项目(2006C13012)
关键词
铁线莲属
药用植物
地上部分
总黄酮
系统演化
Clematis L
Medicinal plants
Aerial parts
Total flavonoids
Phylogenetic correlation
