摘要
利用二苯代苦味酰基自由基体系、H2O2/Fe2+产生的羟基自由基体系、超氧阴离子自由基体系对玉米须黄酮粗提物、乙酸乙酯与正丁醇萃取物的体外清除自由基的能力进行研究,并以芦丁的测定值为对照。试验结果为:清除DPPH自由基的能力依次为:玉米须黄酮粗提物>正丁醇萃取物>乙酸乙酯萃取物。各试样达到最大清除率的浓度及清除率分别为:1、2、2g/L及93%、78%和71%;清除羟自由基的能力依次为:玉米须黄酮粗提物>乙酸乙酯萃取物>正丁醇萃取物,当试样浓度为20g/L时,清除率达到最大,依次为92.04%、82.6%和56.5%;清除超氧阴离子自由基的能力依次为:玉米须黄酮粗提物>乙酸乙酯萃取物>正丁醇萃取物,当试样的浓度为20g/L时,清除率达到最大,依次为94.8%、90%和58%。结果表明,各试样清除羟自由基和超氧阴离子自由基的效果优于芦丁的效果,玉米须黄酮具有较好的清除自由基的能力。
The scavenging activity of free radicals of flavonoids from corn silk were studied and compared with lutin＇s by used the assay of DPPH, H2O2/Fe^2＋ for hydroxyl radical system and superoxide anion radical system. The results showed that the scavenging on DPPH was as fellows： crude extract 〉 crude extract for n-Butanol 〉 crude extract for Ethyl acetate, when the concentration of sample were 1,2 g/L and 2 g/L, the rate of scavenging were 93 %,71% and 78 % respectively. The scavenging on hydroxyl radical was as fellows： crude extract 〉crude extract for Ethyl acetate 〉crude extract for n-Butanol, when the concentration of sample were 2 g/L, the highest scavenging were reached with 92.04 %,82.6% and 56.5% respectively. The scavenging on superoxide anion radical was as fellows： crude extract 〉crude extract for Ethyl acetate 〉crude extract for n-Butanol, when the concentration of sample were 20 g/L, the highest scavenging were reached with 94.8 %,58 % and 92.04 % respectively. The scavenging activity of sample on free radicals were higher than that of lutin＇s. These results suggested that corn silk flavonoids have better capacity of the scavenging free radicals.
出处
《食品研究与开发》
CAS
北大核心
2009年第1期5-8,共4页
Food Research and Development
基金
天津市科技攻关项目(06YFGPNC03800)
关键词
玉米须黄酮
DPPH
羟自由基
超氧阴离子
corn silk flavonoid
DPPH
hydroxyl radical
superoxide anion radical
