摘要
根据Ｎ－乙酰－Ｌ－半胱氨酸与氯化钯（ＰｄＣｌ２）反应，生成一种稳定的黄色化合物，我们建立了比色测定复合结晶氨基酸注射液中的ＮＡＣ的方法。测定波长为３７５ｎｍ，检测结果重现性好，变异系数是３．９％，回收率范围在９３．１％～１００．２％之间，检测下限为０．１６３μｇ／ｍｌ。该方法比经典的电量滴定法灵敏度高、精确性好，较高压液相色谱法操作简便、费用低。
A colorimetric determination of N acetylcysteine(NAC) in crystalline amino acid solution was developed,based on the fact that NAC reacts with palladium chloride(PdCl 2) and forms a stable yellow complex which could be colorimetrically determined at wavelength of 375nm. The coefficient of variation was 3.9%. The yield of recovery was 93.1%～100.2%, and the lowest detection limits was 0.163μg/ml. The method had good sensitivity and accuracy compared with coulometric titration. The method was simpler and more economical compared with high-perfomance liquid chromatography.
出处
《氨基酸和生物资源》
CAS
1999年第2期4-6,共3页
Amino Acids & Biotic Resources
