摘要
目的：研究川芎嗪治疗增生性瘢痕的机制．方法：对增生性瘢痕成纤维细胞进行体外培养，通过ＭＴＴ法检测成纤维细胞的增殖活性，用羟脯氨酸（ＨＰ）比色法测定细胞胶原合成含量．结果：ＭＴＴ法显示川芎嗪可抑制增生性瘢痕成纤维细胞的增殖活性，可以减少成纤维细胞的胶原合成并使细胞形态学发生改变．结论：川芎嗪可以抑制增生性瘢痕成纤维细胞的增殖及胶原合成．
AIM: To investigate the mechanism of the effect of TL on hypertrophic scar fibroblast. METHODS: Hypertrophic scar derived fibroblasts were cultured in vitro . The proliferation of the fibroblasts is examined by MTT method, and the synthesis of collagen by the fibroblasts is examined by hydroxy proline (HP)colorimetric analysis. RESULTS: The absorbance of MTT method indicated that TL can inhibit the proliferation of hypertrophic scar derived fibroblasts. TL can also reduce the collagen synthesis of the cells, when the fibroblasts are treated with TL. CONCLUSION: TL can inhibit the proliferation and collagen synthesis of the hypertrophic scar derived fibroblasts.
出处
《第四军医大学学报》
1999年第11期964-965,共2页
Journal of the Fourth Military Medical University
关键词
川芎嗪
瘢痕
成纤维细胞
胶原
tetramethylpyrazine
scar
fibroblast
collagen
