摘要
目的:研究雷公藤甲素对瘢痕成纤维细胞增殖的影响,为临床增生性瘢痕的防治提供科学的实验依据。方法:采用MTT检测雷公藤甲素对体外培养人瘢痕成纤维细胞增殖的影响,LDH检测细胞毒性。Giemsa染色观察细胞形态,RT-PCR检测TGF-β1的表达。结果:10、20和40μg/ml的雷公藤甲素对体外培养的瘢痕成纤维细胞均有抑制效应;雷公藤甲素作用24h后,可使瘢痕成纤维细胞的突出变短、变少,胞核、胞质浓缩;与对照组比较,雷公藤甲素可降低TGF-β1mRNA水平的表达。结论:雷公藤甲素对瘢痕成纤维细胞的增殖有抑制作用;雷公藤甲素降低TGF-β1mRNA的表达。
Objective ：To investigate the effect of triptolide on proliferation of fibroblasts.Methods ：Cell viability were determined by MTT assay.Cell toxicity was determined by lactate dehydrogenase assay.Cell morphology was determined by Giemsa staining.The human mRNA levels of TGF-β1 in fibroblasts were determined by RT-PCR.Results：Compared with control groups, triptolide caused statistically significant inhibition of cell viability（P〈0.01）without cell toxicity（P〉 0.05）Triptolide caused statistically significant decreases in the level of TGF-β1 compared with control groups （P〈0.01）.Conclusion： 1.Triptolide can inhibit proliferation of the fibroblasts in vitro.2.Triptolide caused decreases production of TGF-β1 mRNA in fibroblasts in vitro.
出处
《医学信息（中旬刊）》
2010年第4期753-754,共2页
Medical Information Operations Sciences Fascicule
