摘要
目的探讨肺纤维化相关细胞因子和炎症因子基因多态性与尘肺易感性的关系。方法以确诊的汉族男性壹期尘肺病患者为病例,选择与病例来自同一工作场所、年龄相差不超过5岁、开始接尘时间及累积接尘工龄相差不超过2年的无尘肺病男性接尘工人为对照。采用聚合酶链反应-限制性片断长度多态性方法检测肿瘤坏死因子(TNF)、纤维粘连蛋白(FN)、转化生长因子-β(TGF-β)、白细胞介素-1(IL-1)、IL-6、人类白细胞抗原(HLA)-DRB1和HLA-DQB1的等位基因及基因型,分析其在2组中的分布情况。采用条件logistic回归方程,进行多因素分析。结果单因素分析发现,病例组携带IL-6(-634)CC、FN MspⅠCC、FN HaeⅢb AA和HLA-DRB1*08等位基因频率高于对照组(P<0.05或P<0.01);IL-1α(-889)1/1、TGF-β(-509 CC、+915 GG)、TNF-α(-308)1/1基因型及HLA-DRB1*09、HLA-DQB1*06等位基频率低于对照组(P<0.05或P<0.01);多因素分析发现,IL-1α(-889)1/1和TGF-β(+915)GG为保护因素;HLA-DRB1*08和FN MspⅠCC基因型为危险因素。结论携带IL-6(-634)CC、FN(MspⅠCC、HaeⅢb AA)基因型和HLA-DRB1*08等位基因的接尘者患尘肺病危险性增加;IL-1α(-889)1/1、TGF-β(-509)CC、TGF-β(+915)GG、TNF-α(-308)1/1基因型及HLA-DRB1*09、HLA-DQB1*06等位基因为保护因素。
Objective To explore the relationship between genetic polymorphism of pulmonary fibrosis and the sensibility of pneumoconiosis. Methods 128 male stage I pneumoconiosis patients were selected as the case group. According to 1：1 paired matching method, 128 workers exposed to the same dust workplaces without pneumoconiosis were selected as the control group. The genotypes and alleles of TNF, FN, TGF-β, IL-1, IL-6, HLA-DRB1 and HLA-DQB1 were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique. The multivariate was analyzed by condition logistic regression analysis. Results The frequencies of IL-6 （ - 634 ） CC, FN Msp ⅠCC, FN Hae HI b AA and HLA-DRB1 ＊ 08 in the case group were significantly higher than those of the control （P 〈0. 05 or P 〈0. 01 ）. The frequencies of 1L-1α（-889） 1/1, TGF-β（ -509 CC and ＋ 915 GG ） , TGF-α （ - 308 ） 1/1, HLA-DRB1 ＊ 09 and HLA-DQB1 ＊06 in the case group were significantly lower than those of the control （P 〈 0.05 or P 〈 0.01 ）. The multivariate analysis showed that IL-1α（-889） 1/1 and TGF-β（ ＋ 915） GG were protective factors but HLA-DRB1 ＊ 08 and FN Msp ⅠCC were risk factors. Conclusion IL-6 （ - 634 ） CC, FN （ Msp ⅠCC and Hae Ⅲ b AA） genotypes and HLA-DRB1 ＊ 08 allele are risk factors. IL-1α （ - 889 ） 1/1, TGF-β （ - 509 ） CC, TGF-β （ ＋ 915）GG, TNF-α（ - 308 ） 1/1 genotypes and HLA-DRB1 ＊ 09, HLA-DQB1 ＊ 06 alleles are protective factors. Key words： Pneumooconiosis; Sensibility;Cytokine; Inflammatory factor; Genetic polymorphism
出处
《中国职业医学》
CAS
北大核心
2011年第5期387-391,共5页
China Occupational Medicine
基金
河北省科技攻关计划资助项目(09276195D)
关键词
尘肺病
易感性
细胞因子
炎症因子
基因多态性
Pneumooconiosis
Sensibility
Cytokine
Inflammatory factor
Genetic polymorphism
