摘要
目的建立烟曲霉角膜炎小鼠模型,探讨烟曲霉角膜炎的形成条件和致病机制。方法采用划痕法和角膜基质注射法建立烟曲霉角膜炎小鼠模型。造模后观察角膜病变,对病变角膜组织做真菌检查。结果划痕法造模小鼠角膜感染率低,病变轻,易自愈;对免疫受损小鼠采用划痕法感染率100%,病情较重,病变持久;基质注射法造模感染率为100%,病变严重、持久。结论烟曲霉孢子对小鼠角膜的感染率与角膜受损程度及黏附的孢子数量呈正比;宿主免疫受损,易诱发烟曲霉角膜炎且病变较重。
Objective To establish a murine model of corneal aspergillosis and investigate the formation condition and pathogenic mechanism of corneal aspergillosis.Methods Murine model of corneal aspergillosis was set up by corneal scarification and intrastromal injection.Morphological observation,histopathological study and fungal examinations（light microscope and culture） were performed afterwards.Results The infection rate was 100% in immunocompetent mice by introstromal injection method.Mild changes which could be self-recovered were observed on the infected corneas,whereas moderate to severe keratomycosis developed in cyclophosphamide-treated mice with the same inoculation.Conclusions The infection rate of Aspergillus fumigatus to murine corneal is related to the damage level of corneal and the amount of spores adhered.Immudeficiency may cause corneal aspergillosis and make the lesion more serious.
出处
《中国真菌学杂志》
2011年第3期141-144,共4页
Chinese Journal of Mycology
