摘要
用改进的ＳＤＳ法提取油梨基因组ＤＮＡ，即在细胞核被裂解之前，先去除细胞质中的酚类物质和蛋白质，然后用ＳＤＳ裂解细胞核，异丙醇和乙醇沉淀ＤＮＡ，成功地从油梨叶片中提取和纯化了ＤＮＡ。以１０个油梨品种的基因组ＤＮＡ为模板，４０个随机引物进行ＲＡＰＤ分析，结果表明，大部分引物可以在不同模板上扩增出条带，但仅有７个引物可以同时在１０个品种的油梨ＤＮＡ上扩增出条带；用ＲＡＰＤ结果对１０个油梨品种进行聚类分析，基本符合传统分类观点。
The genome DNA was successfully extracted from the leaves of ten avocado vareties by using the improved SDS method. The genome DNA of the 10 avocado varieties wasused as templates and amplified with 40 random primers by using RAPD. Results foundthat fragments were amplified on different templates with most of the random primers, whileon all templates only with seven primers. According to the results of RAPD, the geneticrelationship among the 10 varieties was examined for classification of avocado.
出处
《热带作物学报》
CSCD
1999年第4期57-61,共5页
Chinese Journal of Tropical Crops
