摘要
主效抗稻瘟病基因Pita和Pib在我国很多稻区表现高水平的稻瘟病抗性,被广泛应用于我国的水稻育种和生产。但这2个基因在国内品种资源中的分布及利用情况缺乏详细的资料,致使育种利用上存在着盲目性。本研究利用Pita和Pib基因的功能标记,检测和分析了我国115份水稻地方品种的Pita和Pib基因型。结果表明,Pita在我国浙江、福建、广东、广西、贵州、四川、安徽、江西、河南、河北、吉林均有分布,在江苏、上海、山东、湖南、湖北、辽宁没有发现;而只有来自四川的地方品种桂东籼和来自河南的德国稻携带Pib,其他省均未发现。在江苏的主要推广粳稻品种中,晚粳品种几乎都携带这两个基因,部分迟熟中粳携带这两个基因,中熟中粳多数不携带。
The two major blast resistance genes,Pita and Pib,showed broad resistance spectrum to Magnaporthe grisea isolates in many rice planting areas of China and has been utilized in rice breeding and commercial production.However,the distribution of this two genes in present rice germplasms has not been well investigated,resulting in the blindness of their utilization in rice breeding.In the present study,the existence of the two genes in 115 landrace rice germlpasms and 19 rice leading cultivars were rapidly determined using the functional markers of Pita/pita and Pib/pib alleles.The results showed that 20 landrace accessions harbored Pita resistant allele and only 2 landrace accessions harbored Pib resistant allele.The Pita resistant allele distribute widely in china including Zhejiang Province,Fujian Province,Guangdong Province,Guangxi Province,Sichuan Province,Guizhou Province,Anhui Province,Jiangxi Province,Henan Province,Hebei Province,Jilin Province,but not found in Jiangsu Province,Shandong Province,Hunan Province,Hubei Province and Shanghai City.Only two landrace harbored the Pib resistant allele.However,11 cultivars harbored Pita resistant allele and 8 cultivars harbored Pib resistant allele.Interestingly,the 8 cultivars harboring Pib carried the Pita resistant allele simultaneously.These results are useful for rational utilization and deployment of the rice cultivars and germplasm with the Pita and Pib genes and for incorporating and stacking these genes into elite present leading cultivars and lines by marker assisted selection in rice breeding programs nationwide.
出处
《华北农学报》
CSCD
北大核心
2011年第3期1-6,共6页
Acta Agriculturae Boreali-Sinica
基金
国家转基因生物新品种培育科技重大专项(2009ZX08001-005B,019B)
国家科技支撑计划重大项目(2006BADO2A03)
江苏省自然科学基金项目(BK2009321):江苏省农业科技自主创新基金项目
江苏省六大人才高峰项目
江苏省农业科学院基金项目(61107036510806)
