摘要
基因Pi-ta和Pi-b是最早被克隆的两个稻瘟病抗性基因,在粳稻中表现出持久稳定的稻瘟病抗性,因而被广泛用于稻瘟病抗性育种。为明确上述基因在江苏粳稻中的分布,为抗病育种提供依据,本研究利用Pi-ta和Pi-b的功能标记,对40个粳稻品种和665份粳稻新品系进行相关基因型的分子检测。结果表明,抗性基因Pi-ta和Pi-b在江苏粳稻品种中具有一定的分布,其中Pi-b的分布频率高于Pi-ta的频率,连粳系列品种大都不携带Pi-ta和Pi-b抗性基因,而武粳系列品种则基本含有上述抗性基因。粳稻新品系携带抗性基因Pi-ta的频率与推广品种相比变化不大,但携带抗性基因Pi-b的频率明显高于推广品种,这说明人工改良水稻品种有利于抗病基因Pi-b频率的提高。四种基因型中,pi-ta/Pi-b的分布频率最高,为60.0%,其次为Pi-ta/Pi-b,占33.5%,基因型pi-ta/pi-b的分布频率为3.9%,而Pi-ta/pi-b的分布频率最低,只占2.6%。从4个组合的抗性基因来源看,抗性基因Pi-ta则可能来自武香粳14、武粳15或南粳44,而Pi-b则可能来自武粳13、武香粳14、武粳15或南粳44。从4种基因型后代的获得频率看,以南粳44//武粳13/关东194获得抗性基因型Pi-ta/Pi-b后代的频率最高。
Pi-ta and Pi-b, the first cloned rice blast resistant genes, have been widely used in rice blast resistance breeding for their lasting stable resistance. To define the distribution of Pi-ta and Pi-b for japonica rice in Jiangsu, genotypes for resistance genes Pi-ta and Pi-b of 40 varieties and 665 new lines were detected using functional markers of Pi-ta/pi-ta and Pi-b/pi-b alleles. The results exhibited that the resistant allele of Pi-ta and Pi-b was widespread in japonica rice varieties, and the distribution frequency of Pi-b was higher than that of Pi-ta. Most of Lianjing series varieties didn＆#39; t carry the two re-sistance genes, which were different from that of Wujing series varieties. The percentage of resistant allele Pi-ta carried in new lines was similar to that in commercial varie-ties, while the percentage of resistant allele Pi-b was higher than that in commercial varieties. This indicated that artifi-cial selection was beneficial for improving the percentage of＆amp;nbsp;Pi-b allele. In four genotypes, the percentage of pi-ta/Pi-b was the highest, accounting for 60. 0% in 665 new lines, and other three genotypes were Pi-ta/Pi-b, pi-ta/pi-b and Pi-ta/pi-b, accounting for about 33. 5%, 3. 9% and 2. 6% of total, respectively. Judged from the source of resistance genes in four combinantions, the resistant allele Pi-ta might be from parents Wuxiangjing 14, Wujing 15 or Nanjing 44, and Pi-b might come from parents Wujing 13, Wuxiangjing 14, Wujing 15 or Nanjing 44. Analysis the genotype of offspring in these combination, Nanjing 44//Wujing 13/Kantou 194 was the most possi-ble cross combination to yield offsprings carrying Pi-ta/Pi-b.
出处
《江苏农业学报》
CSCD
北大核心
2014年第5期921-927,共7页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业科技自主创新基金项目[CX(12)1003]
现代农业产业技术体系建设专项资金项目(CARS-01-47)
关键词
水稻
稻瘟病
抗性基因
Pi-ta
Pi-b
rice
rice blast
resistance gene
Pi-ta
Pi-b
