摘要
目的了解肺结核患者疱疹病毒感染及免疫功能状态,探讨红细胞免疫功能与T淋巴细胞亚群可能存在的相关性。方法肺结核患者51例,健康体检者30例,采用金标免疫斑点渗滤法检测血浆单纯疱疹病毒(HSV)、EB病毒、人巨细胞病毒(HCMV)Ig M和IgG抗体;酵母菌花环法检测红细胞受体花环率及红细胞免疫复合物花环率;SAP法检测其外周血CD3+T、CD4+T、CD8+T淋巴细胞百分率。结果肺结核患者组HSV-Ig M、EBV-Ig M阳性率分别为88.24%和60.78%,健康对照组分别为66.67%和30.00%,差异均有统计学意义(P<0.05);肺结核患者组HSV-IgG、EBV-IgG、HCMV-Ig M、HCMV-IgG阳性率为80.39%、41.18%、9.80%和39.22%,健康对照组为70.00%、26.70%、0和30.00%,差异均无统计学意义(P>0.05);肺结核患者红细胞C3b受体花环率低于健康对照组(P<0.01),IC花环率高于健康对照组(P<0.01),CD3+、CD4+T淋巴细胞百分率和CD4+/CD8+比值均低于健康对照组(P<0.01)。结论结核病患者存在HSV和EBV的活化感染,其疱疹病毒混合感染可能影响结核病的病情;结核病患者红细胞免疫功能与T淋巴细胞免疫功能均有所下降,可能影响结核病的病情和转归。红细胞免疫功能与T淋巴细胞免疫功能存在正相关。
Objective To learn about herpes virus infection in tuberculosis patients and T-lymphocyte subsets in those patients and to explore the possibie relationship between the immune function of red blood cells and T-lymphocyte subsets. Methods Samples were collected from 51 patients with tuberculosis and 30 normal subjects. A dot-immunogold filtration assay was used to test for IgG and IgM antibodies against HSV, EBV, or HCMV infection. Yeast rosetting was used to measure red blood cell receptors and red blood cell immune complexes. T-lymphocyte subsets were determined using SAP. Results TB patients tested positive for HSV-IgM and EBV-IgM at rates of 88.24% and 60.78% while the healthy control group tested positive at rates of 66.67% and 30.00%. The difference in rates was statistically significant （P〈0. 05）. TB patients tested positive for HSV-IgG, EBV-IgG, HCMV-IgM, and HCMV-IgG at rates of 80.39%, 41.18%, 9.80%, and 39.22% while the healthy control group tested positive at rates of 70.00%, 26.70%, 0%, and 30.00%. The difference in rates was not statistically significant （P〉0.05）. Erythrocyte C3b receptor binding （rosetting） was lower in TB patients than in the control group （P〈0.01）. Immune complex binding was higher in TB patients than in the control group （P〈0.01）. The percentage of CD3＋ and CD4＋ T lymphocytes and CD4＋/CD8＋ ratios were lower in TB patients than in the control group. Conclusion TB patients are more likely to have an active HSV and EBV infection. A mixed infection with herpes virus strains may affect the prevalence of TB. The immune function of red blood cells and T-lymphocyte immunity are diminished in TB patients, and there is a correlation between the immune function of red blood cells and T-lymphocyte immunity.
出处
《中国病原生物学杂志》
CSCD
2011年第6期417-419,共3页
Journal of Pathogen Biology
